Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4368507..4369201 Replicon chromosome
Accession NZ_CP063483
Organism Escherichia coli strain EM03-18-08

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag IQS21_RS20975 Protein ID WP_001263489.1
Coordinates 4368507..4368905 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag IQS21_RS20980 Protein ID WP_000554758.1
Coordinates 4368908..4369201 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 4364095..4364175 - 81 NuclAT_11 - -
- 4364095..4364175 - 81 NuclAT_11 - -
- 4364095..4364175 - 81 NuclAT_11 - -
- 4364095..4364175 - 81 NuclAT_11 - -
IQS21_RS20950 4364771..4365229 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
IQS21_RS20955 4365490..4366947 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
IQS21_RS20960 4367004..4367525 - 522 Protein_4080 peptide chain release factor H -
IQS21_RS20965 4367521..4367727 - 207 Protein_4081 RtcB family protein -
IQS21_RS20970 4368045..4368497 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
IQS21_RS20975 4368507..4368905 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
IQS21_RS20980 4368908..4369201 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
IQS21_RS20985 4369253..4370308 - 1056 WP_001226164.1 DNA polymerase IV -
IQS21_RS20990 4370379..4371164 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
IQS21_RS20995 4371136..4372848 + 1713 Protein_4087 flagellar biosynthesis protein FlhA -
IQS21_RS21000 4373072..4373569 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4343584..4374371 30787


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T178432 WP_001263489.1 NZ_CP063483:c4368905-4368507 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T178432 NZ_CP084023:3575368-3575571 [Pectobacterium aroidearum]
ATGAAAAAAATCGACTATTTGATGCGTTTGCGTAAATGCACAACCATTGACACTCTTGAACGCGTTATTGAAAAAAACAA
GTATGAACTCTCTAATGATGAACTGGAGATGTTCTTTTCCGCAGCCGATCATCGTCTGGCAGAACTGACGATGAACAAAC
TTTACGACAAAGTTCCTACCGCAGTATGGCGATACGTCCGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT178432 WP_000554758.1 NZ_CP063483:c4369201-4368908 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT178432 NZ_CP084023:3574946-3575314 [Pectobacterium aroidearum]
ATGGATGAGTACACACCAAAACATTATGATATTGCCCAACTCAGATTCTTATGTGAGAATCTGTGTGACGAAAGCATAGC
AACGTTAGGCGATAGCAGCCACGGCTGGGTCAATGATCCAACATCTGCGATCAATCTCCAATTAAATGAACTTATTGAGC
ATATCGCTACGTTTATTCTCACATTTAAAATAAAATACCCTAACGAAAGTGAGCTTTCAGAGCAGGTTGAAAAGTATTTG
GATGATACTTACGTCTTGTTTAGCAACTATGGGATTAATGATGCTGAACTGCGGCGCTGGCAGAAGTCCAAAGCAAAATT
ATTCGGAATGTTCTCAGGGGAGAACGTCTGTACGCCTGCTAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References