Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2530518..2531020 Replicon chromosome
Accession NZ_CP063483
Organism Escherichia coli strain EM03-18-08

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IQS21_RS11915 Protein ID WP_000767829.1
Coordinates 2530766..2531020 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IQS21_RS11910 Protein ID WP_001259255.1
Coordinates 2530518..2530769 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS21_RS11885 2525696..2526763 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
IQS21_RS11890 2526763..2527833 - 1071 WP_000108939.1 histidinol-phosphate transaminase -
IQS21_RS11895 2527830..2529134 - 1305 WP_001614652.1 histidinol dehydrogenase -
IQS21_RS11900 2529140..2530039 - 900 WP_000131777.1 ATP phosphoribosyltransferase -
IQS21_RS11905 2530185..2530235 - 51 WP_001364200.1 his operon leader peptide -
IQS21_RS11910 2530518..2530769 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IQS21_RS11915 2530766..2531020 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IQS21_RS11920 2531103..2531927 + 825 WP_000754749.1 SDR family oxidoreductase -
IQS21_RS11925 2531973..2532902 + 930 WP_000803351.1 LysR family transcriptional regulator -
IQS21_RS11930 2533117..2533179 + 63 WP_010723108.1 membrane protein YoeI -
IQS21_RS11935 2533169..2534527 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IQS21_RS11940 2534706..2535764 + 1059 WP_000492321.1 transport protein YeeE -
IQS21_RS11945 2535778..2536005 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T178418 WP_000767829.1 NZ_CP063483:2530766-2531020 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T178418 NZ_CP084009:29018-29284 [Staphylococcus epidermidis]
ATGGCTAAATATAAAATCAACATAAAGAATTCAGCTAAGACAGATTTAAAGAAGATAAAACGTTCTCACTTACAAGAACA
ATTTTTGAAAATCGTAGAAATACTAAAATATGATCCATATCAACAAAGTCAATCATTTGAAAAATTGCACCCTAAACATT
TAGGACGATACTCAAGACGTATCAATCATCAACATAGAGTTGTCTATACAATTGATGAGGAAAATAAAGAAGTATTAATA
TTAGCAGCTTGGTCTCATTATGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT178418 WP_001259255.1 NZ_CP063483:2530518-2530769 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT178418 NZ_CP084009:28761-29018 [Staphylococcus epidermidis]
ATGAAAAGTAAAACGCCAACAGAAGCTCGTAAAGATTTTTATCAGTTATTAAAACGTGTGAATAATGACCATGAACCAGT
ATATATTGAAGGCAAATATGATGAGAGTAATGCTGTGATTATAGGTATGGAAGATTGGCGTAGTATTAAAGAAACTATAT
ATCTTGAAGCTACAGGAACAATGCAAAAAGTTAGAGAACGTGAACAAGACAACAGTGGTTATACAAACATAGATGATATT
GATTGGGCTAGACTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References