Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1334347..1335146 Replicon chromosome
Accession NZ_CP063483
Organism Escherichia coli strain EM03-18-08

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag IQS21_RS06270 Protein ID WP_000347275.1
Coordinates 1334347..1334811 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IQS21_RS06275 Protein ID WP_001307405.1
Coordinates 1334811..1335146 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS21_RS06240 1329348..1329782 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
IQS21_RS06245 1329800..1330678 - 879 WP_039268471.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IQS21_RS06250 1330668..1331447 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IQS21_RS06255 1331458..1331931 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
IQS21_RS06260 1331954..1333234 - 1281 WP_039268470.1 tagatose-bisphosphate aldolase subunit KbaZ -
IQS21_RS06265 1333483..1334292 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IQS21_RS06270 1334347..1334811 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IQS21_RS06275 1334811..1335146 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IQS21_RS06280 1335295..1336866 - 1572 WP_001273752.1 galactarate dehydratase -
IQS21_RS06285 1337241..1338575 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
IQS21_RS06290 1338591..1339361 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T178413 WP_000347275.1 NZ_CP063483:c1334811-1334347 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T178413 NZ_CP084007:1054249-1054521 [Flavonifractor plautii]
ATGTATAACATAAGACCGACCACAAAATTTCAGCGGGATTTGAAGCGCGTAAAAAGGCGTGGCTTTGATATTTCGCTGTT
GACTGACATTATTAAAAAGCTAGCGGCGGGAGAGCCACTGCCAGAGAAGAATAGGGATCACCAGATGTCCGGGGATTATG
CAGGGTGTCGTGAGTGCCACATTACCCCGGATTGGCTCCTCATTTATGAAGTGGACGGGAACGAGCTGATTTTATATCTC
ACCCGGACGGGATCGCATAGCGACTTATTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT178413 WP_001307405.1 NZ_CP063483:c1335146-1334811 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT178413 NZ_CP084007:1053975-1054256 [Flavonifractor plautii]
ATGGCTAACACAAATATCAATATCCGTATGGACACGGATTTGAAAAAGCAGTTTGAAGCGTTCTGTGCAGACATGGGAAT
GACTATGACAACGGCCTTTAACATTTTTGCAAGGAAGGCTGTCCGGGAGTATAGAATACCCTTTGAAATCAGCGGTGATG
TGCCGAACGCTGAAACCGTCGAAGCAATCCAAGAGGTAAAGAGAATGAAAGCCAATCCGAGCCTCGGCAAGACCTATTCC
GATGTCGATCAGATGATAGAGGAGCTGCTTGCCGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References