Detailed information of TA system TA178407

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 57785..58211
• Replicon: plasmid pEM06-18-14_2
• Accession: NZ_CP063481
• Organism: Escherichia coli strain EM06-18-14

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IQS22_RS23975
• Protein ID: WP_001312861.1
• Coordinates: 57785..57943 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	57987..58211 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS22_RS23940	52840..53067	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
IQS22_RS23945	53161..53847	-	687	WP_015059009.1	PAS domain-containing protein	-
IQS22_RS23950	54038..54421	-	384	WP_000124981.1	relaxosome protein TraM	-
IQS22_RS23955	54698..55345	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
IQS22_RS23960	55642..56463	-	822	WP_001234445.1	DUF945 domain-containing protein	-
IQS22_RS23965	56574..56870	-	297	WP_001272251.1	hypothetical protein	-
IQS22_RS23970	57170..57547	+	378	Protein_64	hypothetical protein	-
IQS22_RS23975	57785..57943	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	57987..58211	-	225	NuclAT_0	-	Antitoxin
-	57987..58211	-	225	NuclAT_0	-	Antitoxin
-	57987..58211	-	225	NuclAT_0	-	Antitoxin
-	57987..58211	-	225	NuclAT_0	-	Antitoxin
IQS22_RS23980	58023..58202	+	180	WP_001309233.1	hypothetical protein	-
IQS22_RS23985	58223..58942	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
IQS22_RS23990	58939..59373	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IQS22_RS23995	59442..61465	-	2024	Protein_69	ParB/RepB/Spo0J family partition protein	-
IQS22_RS24000	61526..61759	-	234	WP_000006003.1	DUF905 family protein	-
IQS22_RS24005	61817..62344	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
IQS22_RS24010	62646..63101	+	456	WP_001424978.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / aadA2 / dfrA12 / blaTEM-1B / rmtB / blaNDM-5	-	1..91066	91066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T178407 WP_001312861.1 NZ_CP063481:c57943-57785 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T178407 NZ_CP084001:c4038403-4038119 [Salmonella sp. A7]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT178407 NZ_CP063481:c58211-57987 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin