Detailed information of TA system TA178403

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 18359..18612
• Replicon: plasmid pEM06-18-14_2
• Accession: NZ_CP063481
• Organism: Escherichia coli strain EM06-18-14

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: IQS22_RS23745
• Protein ID: WP_001312851.1
• Coordinates: 18359..18508 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	18553..18612 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS22_RS23720	14076..14693	+	618	WP_000521604.1	hypothetical protein	-
IQS22_RS23725	14752..15744	-	993	WP_001579760.1	hypothetical protein	-
IQS22_RS23730	16658..17515	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
IQS22_RS23735	17508..17582	-	75	WP_001375168.1	RepA leader peptide Tap	-
IQS22_RS23740	17827..18075	-	249	WP_000083837.1	replication regulatory protein RepA	-
IQS22_RS23745	18359..18508	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	18553..18612	+	60	NuclAT_1	-	Antitoxin
-	18553..18612	+	60	NuclAT_1	-	Antitoxin
-	18553..18612	+	60	NuclAT_1	-	Antitoxin
-	18553..18612	+	60	NuclAT_1	-	Antitoxin
IQS22_RS23750	18813..19043	-	231	WP_001736714.1	hypothetical protein	-
IQS22_RS23755	19207..19806	-	600	WP_032083981.1	hypothetical protein	-
IQS22_RS23760	20192..20392	-	201	WP_015059022.1	hypothetical protein	-
IQS22_RS23765	20524..21084	-	561	WP_000139328.1	fertility inhibition protein FinO	-
IQS22_RS23770	21139..21885	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / aadA2 / dfrA12 / blaTEM-1B / rmtB / blaNDM-5	-	1..91066	91066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T178403 WP_001312851.1 NZ_CP063481:c18508-18359 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T178403 NZ_CP084001:2002180-2002283 [Salmonella sp. A7]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 60 bp

>AT178403 NZ_CP063481:18553-18612 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin