Detailed information of TA system TA178402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4782534..4783333
• Replicon: chromosome
• Accession: NZ_CP063479
• Organism: Escherichia coli strain EM06-18-14

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F4VJD3
• Locus tag: IQS22_RS22935
• Protein ID: WP_000347266.1
• Coordinates: 4782869..4783333 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: IQS22_RS22930
• Protein ID: WP_001307405.1
• Coordinates: 4782534..4782869 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS22_RS22915	4778319..4779089	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
IQS22_RS22920	4779105..4780439	-	1335	WP_000599640.1	galactarate/glucarate/glycerate transporter GarP	-
IQS22_RS22925	4780814..4782385	+	1572	WP_001273741.1	galactarate dehydratase	-
IQS22_RS22930	4782534..4782869	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
IQS22_RS22935	4782869..4783333	+	465	WP_000347266.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
IQS22_RS22940	4783388..4784197	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
IQS22_RS22945	4784446..4785726	+	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
IQS22_RS22950	4785749..4786222	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
IQS22_RS22955	4786233..4787012	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
IQS22_RS22960	4787002..4787880	+	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
IQS22_RS22965	4787898..4788332	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4771878..4783333	11455

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T178402 WP_000347266.1 NZ_CP063479:4782869-4783333 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T178402 NZ_CP084001:821309-821722 [Salmonella sp. A7]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CGCGCCAACACTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT178402 WP_001307405.1 NZ_CP063479:4782534-4782869 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT178402 NZ_CP084001:821062-821328 [Salmonella sp. A7]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A836NGD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7