Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3547606..3548108 Replicon chromosome
Accession NZ_CP063479
Organism Escherichia coli strain EM06-18-14

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IQS22_RS17115 Protein ID WP_000767829.1
Coordinates 3547606..3547860 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A2S1Y4D6
Locus tag IQS22_RS17120 Protein ID WP_001259254.1
Coordinates 3547857..3548108 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS22_RS17085 3542621..3542848 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
IQS22_RS17090 3542862..3543920 - 1059 WP_024193414.1 transport protein YeeE -
IQS22_RS17095 3544099..3545457 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IQS22_RS17100 3545447..3545509 - 63 WP_010723108.1 membrane protein YoeI -
IQS22_RS17105 3545724..3546653 - 930 WP_000803351.1 LysR family transcriptional regulator -
IQS22_RS17110 3546699..3547523 - 825 WP_000754737.1 SDR family oxidoreductase -
IQS22_RS17115 3547606..3547860 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IQS22_RS17120 3547857..3548108 - 252 WP_001259254.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IQS22_RS17125 3548391..3548441 + 51 WP_001364200.1 his operon leader peptide -
IQS22_RS17130 3548587..3549486 + 900 WP_072645750.1 ATP phosphoribosyltransferase -
IQS22_RS17135 3549492..3550796 + 1305 WP_001345878.1 histidinol dehydrogenase -
IQS22_RS17140 3550793..3551863 + 1071 WP_072645751.1 histidinol-phosphate transaminase -
IQS22_RS17145 3551863..3552930 + 1068 WP_000080109.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T178397 WP_000767829.1 NZ_CP063479:c3547860-3547606 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T178397 NZ_CP083990:c4130789-4130616 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9292.57 Da        Isoelectric Point: 4.5822

>AT178397 WP_001259254.1 NZ_CP063479:c3548108-3547857 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGIGTEKD
IIE

Download         Length: 252 bp

>AT178397 NZ_CP083990:c4131113-4130817 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A2S1Y4D6

References