Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1632456..1632997 Replicon chromosome
Accession NZ_CP063479
Organism Escherichia coli strain EM06-18-14

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag IQS22_RS07835 Protein ID WP_000615982.1
Coordinates 1632456..1632734 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag IQS22_RS07840 Protein ID WP_000729704.1
Coordinates 1632737..1632997 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS22_RS07820 1630038..1630616 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
IQS22_RS07825 1630822..1631589 + 768 WP_000333380.1 class II glutamine amidotransferase -
IQS22_RS07830 1631560..1632300 - 741 WP_001225679.1 murein L,D-transpeptidase -
IQS22_RS07835 1632456..1632734 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IQS22_RS07840 1632737..1632997 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IQS22_RS07845 1633183..1633956 + 774 WP_000543899.1 C40 family peptidase -
IQS22_RS07850 1634013..1634369 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
IQS22_RS07855 1634362..1634640 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
IQS22_RS07860 1634745..1636457 - 1713 Protein_1521 flagellar biosynthesis protein FlhA -
IQS22_RS07865 1636429..1637214 + 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T178384 WP_000615982.1 NZ_CP063479:c1632734-1632456 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T178384 NZ_CP083990:2233862-2234149 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT178384 WP_000729704.1 NZ_CP063479:c1632997-1632737 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT178384 NZ_CP083990:2233623-2233862 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References