Detailed information of TA system TA178380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 721405..722007
• Replicon: chromosome
• Accession: NZ_CP063479
• Organism: Escherichia coli strain EM06-18-14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IQS22_RS03515
• Protein ID: WP_000897305.1
• Coordinates: 721405..721716 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IQS22_RS03520
• Protein ID: WP_000356397.1
• Coordinates: 721717..722007 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS22_RS03485	716435..717220	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
IQS22_RS03490	717319..717918	+	600	WP_001295269.1	glucose-1-phosphatase	-
IQS22_RS03495	717912..718784	+	873	WP_000920762.1	virulence factor BrkB family protein	-
IQS22_RS03500	718781..719218	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
IQS22_RS03505	719263..720204	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
IQS22_RS03510	720268..721176	-	909	WP_001162704.1	alpha/beta hydrolase	-
IQS22_RS03515	721405..721716	+	312	WP_000897305.1	hypothetical protein	Toxin
IQS22_RS03520	721717..722007	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
IQS22_RS03525	722593..722811	+	219	WP_001611167.1	ribbon-helix-helix domain-containing protein	-
IQS22_RS03530	723030..723272	+	243	WP_001086388.1	hypothetical protein	-
IQS22_RS03535	723602..724531	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
IQS22_RS03540	724528..725163	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
IQS22_RS03545	725160..726062	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T178380 WP_000897305.1 NZ_CP063479:721405-721716 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T178380 NZ_CP063479:721405-721716 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT178380 WP_000356397.1 NZ_CP063479:721717-722007 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT178380 NZ_CP063479:721717-722007 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7