Detailed information of TA system TA178373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4327164..4327963
• Replicon: chromosome
• Accession: NZ_CP063476
• Organism: Escherichia coli strain EM10-18-28

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: IQS23_RS20770
• Protein ID: WP_000347273.1
• Coordinates: 4327164..4327628 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: IQS23_RS20775
• Protein ID: WP_001308975.1
• Coordinates: 4327628..4327963 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS23_RS20740	4322165..4322599	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
IQS23_RS20745	4322617..4323495	-	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
IQS23_RS20750	4323485..4324264	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
IQS23_RS20755	4324275..4324748	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
IQS23_RS20760	4324771..4326051	-	1281	WP_023908831.1	tagatose-bisphosphate aldolase subunit KbaZ	-
IQS23_RS20765	4326300..4327109	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
IQS23_RS20770	4327164..4327628	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
IQS23_RS20775	4327628..4327963	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
IQS23_RS20780	4328112..4329683	-	1572	WP_023908830.1	galactarate dehydratase	-
IQS23_RS20785	4330058..4331392	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
IQS23_RS20790	4331408..4332178	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T178373 WP_000347273.1 NZ_CP063476:c4327628-4327164 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T178373 NZ_CP083990:c608432-607968 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT178373 WP_001308975.1 NZ_CP063476:c4327963-4327628 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT178373 NZ_CP083990:c608767-608432 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08