Detailed information of TA system TA178371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3464368..3464970
• Replicon: chromosome
• Accession: NZ_CP063476
• Organism: Escherichia coli strain EM10-18-28

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IQS23_RS16690
• Protein ID: WP_000897305.1
• Coordinates: 3464659..3464970 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IQS23_RS16685
• Protein ID: WP_000356397.1
• Coordinates: 3464368..3464658 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS23_RS16665	3460870..3461772	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
IQS23_RS16670	3461769..3462404	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
IQS23_RS16675	3462401..3463330	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
IQS23_RS16680	3463545..3463763	-	219	WP_203038286.1	ribbon-helix-helix domain-containing protein	-
IQS23_RS16685	3464368..3464658	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
IQS23_RS16690	3464659..3464970	-	312	WP_000897305.1	hypothetical protein	Toxin
IQS23_RS16695	3465199..3466107	+	909	WP_001318161.1	alpha/beta hydrolase	-
IQS23_RS16700	3466171..3467112	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
IQS23_RS16705	3467157..3467594	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
IQS23_RS16710	3467591..3468463	-	873	WP_000920762.1	virulence factor BrkB family protein	-
IQS23_RS16715	3468457..3469056	-	600	WP_001314324.1	glucose-1-phosphatase	-
IQS23_RS16720	3469155..3469940	-	786	WP_000059679.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T178371 WP_000897305.1 NZ_CP063476:c3464970-3464659 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T178371 NZ_CP083975:c479192-478902 [Rhodococcus rhodochrous]
GTGAGCGATAATCCTGCGGCCCGGCCCGGTCCGTATCACGTCGAAGTCGCGTCCCCGGCACGCCGCGACCTCGGACGACT
CCCGCCACGAATCGTCCACGCCGTCATCGAGTTCATCTCCGGCCCCCTCGCCGACAATCCGCACCGGCTCAGCAAACCGC
TGCGCGACGACCTCGAAGGTCTCCACAGCGCACGCCGTGGCGACTACCGCATCCTGCTGCGCATCGACGACCCCAACCAC
ACCATCGTCATCGTGAAAATCGACCACCGAGCCCACGCCTACCGCACCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT178371 WP_000356397.1 NZ_CP063476:c3464658-3464368 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT178371 NZ_CP083975:c479464-479189 [Rhodococcus rhodochrous]
ATGACGACAGTGCCACTCGGCGAAGCCAAGGACAAGCTCTCCGCGCTGGTCGACAGCGCCGAGACCACGCACGACATCAT
CACGATCACCAAACACGGCAAACCTGCCGCGGTGCTGATGTCGGCTGATGACCTCGAATCGCTGCACGAGACGATCTACT
GGCTCTCACGTGCCGACACCCGCGACGCCGTCGCGGCCGCCGACGCCGAGTACGCCGCCGGTCAGACCATCTCCCTCGAG
GAACTGCGCGCCGAACACGGCATGCCGTCCAAGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7