Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2582810..2583504 Replicon chromosome
Accession NZ_CP063476
Organism Escherichia coli strain EM10-18-28

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag IQS23_RS12605 Protein ID WP_001263491.1
Coordinates 2582810..2583208 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag IQS23_RS12610 Protein ID WP_000554755.1
Coordinates 2583211..2583504 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS23_RS12575 2578072..2579529 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
IQS23_RS12580 2579538..2579819 + 282 WP_022645226.1 hypothetical protein -
IQS23_RS12585 2579836..2580345 - 510 WP_001361775.1 hydrolase -
IQS23_RS12590 2580407..2581021 - 615 WP_022645225.1 peptide chain release factor H -
IQS23_RS12595 2581018..2582157 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
IQS23_RS12600 2582348..2582800 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
IQS23_RS12605 2582810..2583208 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
IQS23_RS12610 2583211..2583504 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
IQS23_RS12615 2583556..2584611 - 1056 WP_022645222.1 DNA polymerase IV -
IQS23_RS12620 2584682..2585467 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
IQS23_RS12625 2585439..2587151 + 1713 Protein_2477 flagellar biosynthesis protein FlhA -
IQS23_RS12630 2587249..2588022 - 774 WP_022645219.1 C40 family peptidase -
IQS23_RS12635 2588208..2588468 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T178367 WP_001263491.1 NZ_CP063476:c2583208-2582810 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T178367 NZ_CP083957:43596-44123 [Mesorhizobium sp. B1-1-8]
GTGAGCCTTCCGCCCTGGCATGAGGAGGCGATCGCCCGGTCGCACGACCGCAAAGCCTTTGACTGCGGCGATGTGGAGAT
GAATGCCTTCCTGCAGCGGTTCGCGCGACAAGGGCACGAGCAGAACGCGGTAAAGACGTTCTGTGCGGTTCCCGACGATG
CACCGGACAAAATCCTTGGCTTCTACAGCCTGGCTCCAGCCTCGGTCGAACATCACGCAGTTCCTGCCGCGATGACGAAG
GGTCTGGCGCGACACGATGTGCCGGAATTTCTGCTGGCACGGCTCGCCGTCGACAAATCGGTGGCGGGCAAGGGGCTCGG
TGGTCAGCTTCTGCTGGCAGCCGCGCTGCGCTGCATCCGCGTTACTGAAGAGGTCGGCGGTGTGCTGATGATCATCGACG
CCAAGAGCAAGCGGGCGGCGAACTGGTACGCGAGCTACGGCGCCGAGCCGCTGAATGATCGTCCAATGACGCTTGTCGTT
CCGCTCAAGACCTTTGCCGAGACCTTGCAAGCCAGCGGGCACCTCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT178367 WP_000554755.1 NZ_CP063476:c2583504-2583211 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT178367 NZ_CP083957:43321-43599 [Mesorhizobium sp. B1-1-8]
ATGGCGCGGGCAGCCCTTGATGATACTGATCGGATGAACATCCGGGTGAAGCCGGAGGTGAAGGCGCGCCTGCTGCGCGC
CGCAGCGCTTCGCCATACCGATCTCACCAGCTTCGTCACCCAGTCCGCGCTGCGCGAAGCCGAAACCGTTATCGCCGAAG
CTGACGCCATAAAGGTGTCCGAGCGGGATTTTGGCCGCATCCTGGAGCTTCTCGACAATCCTCCAAAGCCGAACGCGAGG
TTGCGCGCTGCGGCGGCCGCACTTCCGAAGAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References