Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 2359058..2359737 Replicon chromosome
Accession NZ_CP063476
Organism Escherichia coli strain EM10-18-28

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag IQS23_RS11560 Protein ID WP_000057541.1
Coordinates 2359435..2359737 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag IQS23_RS11555 Protein ID WP_000806442.1
Coordinates 2359058..2359399 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS23_RS11545 2355301..2356233 - 933 WP_022645289.1 glutaminase A -
IQS23_RS11550 2356496..2359000 + 2505 WP_038431953.1 copper-exporting P-type ATPase CopA -
IQS23_RS11555 2359058..2359399 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
IQS23_RS11560 2359435..2359737 - 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IQS23_RS11565 2359870..2360664 + 795 WP_063814887.1 TraB/GumN family protein -
IQS23_RS11570 2360868..2361347 + 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
IQS23_RS11575 2361384..2363036 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
IQS23_RS11580 2363254..2364474 + 1221 WP_022645286.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T178365 WP_000057541.1 NZ_CP063476:c2359737-2359435 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T178365 NZ_CP083956:4298623-4298811 [Mesorhizobium sp. B1-1-8]
ATGAAGAGCGCGGATGTCATCGACAGTTTGTTGGCCGATGGCTGGTTCGAGGTGGGTCGCAAGGGCAGTCATGCCCAGTT
CAAACATCGTCGCAAACCGGGCCGGGTGACCGTCCCGCATCCCAAGCGCGACATCCCGATCGGCACGCTGAAAAGCATCG
AGAAGCAATCCGGCCTGAAGCTGAGGTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT178365 WP_000806442.1 NZ_CP063476:c2359399-2359058 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT178365 NZ_CP083956:4298816-4299217 [Mesorhizobium sp. B1-1-8]
ATGCGTCACTATATCGGACTGATTCACAAGGAAGCCGACAGCGATTTCGGCGTCTCCTTCCCTGACTTTCCGGGCGTGGT
CACGGCCGGCACGACCCTTGATGATGCCCGCGCCATGGCAGAGGAGGCGCTGGCCTTTCACGTCGACGGCCTTGTCGAGG
ATGGTGAAGCCATTCCCGAGCCGTCATCCCTCGATGACGTGATGGCCGACAGCGACAACCGCATGGGCGTAGCCATCCTG
GTCGCCCTGAAAGCCGAAGCGGCGAAGGTCGTGCGCGTCAACGTCTCCATTCCGGAGGACGTGCTCAGCGAAATCGACCG
CTATGCCGAACGCCACGGCTACAGCCGTTCCGGGTTCCTGACTGCGGCCGCCAAGCAGGCGATGCGTAACGAGGCCGCCT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References