Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 672765..673267 Replicon chromosome
Accession NZ_CP063476
Organism Escherichia coli strain EM10-18-28

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IQS23_RS03130 Protein ID WP_000767829.1
Coordinates 673013..673267 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IQS23_RS03125 Protein ID WP_001259255.1
Coordinates 672765..673016 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS23_RS03100 667943..669010 - 1068 WP_001563933.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
IQS23_RS03105 669010..670080 - 1071 WP_001682999.1 histidinol-phosphate transaminase -
IQS23_RS03110 670077..671381 - 1305 WP_042031741.1 histidinol dehydrogenase -
IQS23_RS03115 671387..672286 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
IQS23_RS03120 672432..672482 - 51 WP_001364200.1 his operon leader peptide -
IQS23_RS03125 672765..673016 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IQS23_RS03130 673013..673267 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IQS23_RS03135 673350..674174 + 825 WP_000754749.1 SDR family oxidoreductase -
IQS23_RS03140 674220..675149 + 930 WP_000803351.1 LysR family transcriptional regulator -
IQS23_RS03145 675364..675426 + 63 WP_010723108.1 membrane protein YoeI -
IQS23_RS03150 675416..676774 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IQS23_RS03155 676953..678011 + 1059 WP_000492315.1 transport protein YeeE -
IQS23_RS03160 678025..678252 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T178352 WP_000767829.1 NZ_CP063476:673013-673267 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T178352 NZ_CP083952:c4525041-4524766 [Mesorhizobium sp. B2-1-8]
ATGGAGCTGAAGTGGACCAGTAAGGCTTTGTCCGATCTGGTCAGGCTTCATGACTTTCTTGCAGCAGTGAACAATCAGGC
GGCAGCCCGTGCGGTGCAGTCGCTGGTTGCCGCCCCAGCACGGCTAATCGAGAATCCGCGTATCGGGGAGAAGCTGGAAG
AGTTCGAGCCGCGCGAAGTCCGCCGCATTTTGGTCGGCAACTACGAGATTCGCTATGAGATAGCGAACGCCACGATTCTG
TTGCTGCGCCTGTGGCACACGCGAGAGGATCGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT178352 WP_001259255.1 NZ_CP063476:672765-673016 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT178352 NZ_CP083952:c4525301-4525041 [Mesorhizobium sp. B2-1-8]
ATGGACACAAAAGTTATCACCGCTCATGTTCCGCTACCGCTTGCGGAGAAAGTGGACCAACTGGCTGCTCGACTTGAGCG
TCCGCGCGGGTGGATCGTCAAACAAGCCCTTTCCGCTTGGGTCGATTTGGAGGAGGAACGTCGACGGCTCACGCTTGAAG
CGCTGGCCGATGTCGACGCTGGTCGGGTTATAGACCACCAGGCCGTTCAGGCATGGGCCGAGAGTCTTGGTACTGAAAAG
CCACTGCCGGTACCACGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References