Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3871870..3872597 Replicon chromosome
Accession NZ_CP063473
Organism Escherichia coli strain EM18-18-42

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag IQS24_RS18685 Protein ID WP_000550189.1
Coordinates 3872283..3872597 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IQS24_RS18680 Protein ID WP_000560266.1
Coordinates 3871870..3872286 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS24_RS18670 3867030..3869381 + 2352 WP_053295378.1 alpha-glucosidase -
IQS24_RS18675 3869807..3871825 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
IQS24_RS18680 3871870..3872286 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
IQS24_RS18685 3872283..3872597 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
IQS24_RS18690 3872881..3874017 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
IQS24_RS18695 3874102..3874605 + 504 WP_001295542.1 M48 family metallopeptidase -
IQS24_RS18700 3874682..3875365 + 684 WP_001183046.1 vancomycin high temperature exclusion protein -
IQS24_RS18705 3875444..3876430 + 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T178350 WP_000550189.1 NZ_CP063473:c3872597-3872283 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T178350 NZ_CP083952:c3096662-3096366 [Mesorhizobium sp. B2-1-8]
GTGATCGTCGGGTTTCGGGACGGATGGCTACGGGCCTTCTTCGTCGAAGACATTCACTCCCGCAACATTCCGTCCGACCT
TGAAAGCCGGTTATTTCGCAAGCTCCAGATGATCGATGACGCGATGACCGATCTGGATTTGCGTGTGCCACCCAGCAACC
ATTTCGAGAAGTTGCGCGGCAATCTCGATGGCTTCCATTCGATCCGCGTCAACAGCCAATGGCGGCTGATCTTCCGGTGG
GACGGCGGACGCGGTGAAGCGTCGGGTATTTATCTCGACGACCATAGTTACAGATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT178350 WP_000560266.1 NZ_CP063473:c3872286-3871870 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT178350 NZ_CP083952:c3096357-3096049 [Mesorhizobium sp. B2-1-8]
ATGTTGATGACCACACGCAAGCCTGCAACAGTTGGCGAAATCCTCACGGAAGAATTCATGCAACCGCTTGGCCTGACACA
GGCGGCTTTGGCCGAAGCGATGGGGGTCCAGCGCAAGCATGTGAACGAATTGTGCAACGACCGCCGTAACGTGACGGCTG
CGACCGCGCTTATTCTGGCGCGGGTCTTCGGCAACAGCCCGGATTTCTGGCTGAACGTGCAGCGCCGTAGCGATCTCTGG
TCGGTCATGAACTCGCCCGACGAGCGCGCGCGCGTCGATCGCGCCAAGCCTTTGGCGACTGCGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References