Detailed information of TA system TA178346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2779036..2779538
• Replicon: chromosome
• Accession: NZ_CP063473
• Organism: Escherichia coli strain EM18-18-42

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: IQS24_RS13640
• Protein ID: WP_000767829.1
• Coordinates: 2779036..2779290 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: IQS24_RS13645
• Protein ID: WP_001259255.1
• Coordinates: 2779287..2779538 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS24_RS13615	2774301..2775272	-	972	WP_170386723.1	IS30-like element ISApl1 family transposase	-
IQS24_RS13620	2775529..2776887	-	1359	WP_000019201.1	putrescine/proton symporter PlaP	-
IQS24_RS13625	2776877..2776939	-	63	WP_010723108.1	membrane protein YoeI	-
IQS24_RS13630	2777154..2778083	-	930	WP_000803351.1	LysR family transcriptional regulator	-
IQS24_RS13635	2778129..2778953	-	825	WP_000754763.1	SDR family oxidoreductase	-
IQS24_RS13640	2779036..2779290	-	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
IQS24_RS13645	2779287..2779538	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
IQS24_RS13650	2779821..2779871	+	51	WP_001364200.1	his operon leader peptide	-
IQS24_RS13655	2780017..2780916	+	900	WP_000131776.1	ATP phosphoribosyltransferase	-
IQS24_RS13660	2780922..2782226	+	1305	WP_001317939.1	histidinol dehydrogenase	-
IQS24_RS13665	2782223..2783293	+	1071	WP_205595419.1	histidinol-phosphate transaminase	-
IQS24_RS13670	2783293..2784360	+	1068	WP_086624491.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2774301..2775224	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T178346 WP_000767829.1 NZ_CP063473:c2779290-2779036 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T178346 NZ_CP083952:c1208500-1208246 [Mesorhizobium sp. B2-1-8]
ATGAACTGGCACCTTCAAGACGCAAAAAACAATTTCTCCAAGCTGGTCCAACGCGCGCGCAGCGAGGGACCGCAGACGGT
AACTCTGCGCGGCGAGCGTGCCGCAGTGGTACTCTCCGCCGAGGATTACGACCGTCTGGCTGGAAAAAAGAAGTCGTTGG
CTGAGTATCTTTTGACTGGCCCGGTTTGGGACGACGATTTCGCCGAGGAGGTCAACCGTCGCCCGGAAACGATGATCCGG
AACGTCGATCTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT178346 WP_001259255.1 NZ_CP063473:c2779538-2779287 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT178346 NZ_CP083952:c1208246-1207845 [Mesorhizobium sp. B2-1-8]
ATGTATCTGCTCGATACGAACATCGTTTCCGATGTGCAGAGGCGGCTGCATAGGCCGACTGAATGGCTGGCTTCGGTGGA
TCCAACATCGGTCAACCTCAGCGTCATTACCCTTGGTGAAATCGAGCGTGGGATCGTCAAGCTGGGCAAGGTCGATTCCG
AAAAGGCGACACGCCTCGATTTATGGCTACGGGAGCTTCGGCGTGACAACGCCGACCGTATCCTTGCTGTCACGGAAGAC
GTGGCATTGACTTGGGACCGCATCACCGCCGGTCGGACACGAGGCAGCGCTGACACGCTCATCGCGGCAACCGCACTTGT
CCATGACCTCATTCTGGTAACTCGCAATGTCACAGACTTTGAAGATACTGGCGTGACGGTGCTCAATCCCTGGGACGTCT
AA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0