Detailed information of TA system TA178332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 885822..886516
• Replicon: chromosome
• Accession: NZ_CP063473
• Organism: Escherichia coli strain EM18-18-42

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IQS24_RS04155
• Protein ID: WP_001263493.1
• Coordinates: 886118..886516 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IQS24_RS04150
• Protein ID: WP_000554757.1
• Coordinates: 885822..886115 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS24_RS04130	881462..881959	+	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-
IQS24_RS04135	882175..883887	-	1713	Protein_794	flagellar biosynthesis protein FlhA	-
IQS24_RS04140	883859..884644	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
IQS24_RS04145	884715..885770	+	1056	WP_085452615.1	DNA polymerase IV	-
IQS24_RS04150	885822..886115	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IQS24_RS04155	886118..886516	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IQS24_RS04160	886526..886747	+	222	Protein_799	GNAT family N-acetyltransferase	-
IQS24_RS04165	886764..887461	-	698	Protein_800	IS1 family transposase	-
IQS24_RS04170	887522..887755	+	234	Protein_801	GNAT family N-acetyltransferase	-
IQS24_RS04175	888001..888204	+	204	Protein_802	RNA ligase RtcB family protein	-
IQS24_RS04180	888203..888724	+	522	Protein_803	peptide chain release factor H	-
IQS24_RS04185	888781..890238	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
IQS24_RS04190	890499..890957	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	871268..888724	17456
	-	flank	IS/Tn	-	-	886764..887141	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T178332 WP_001263493.1 NZ_CP063473:886118-886516 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T178332 NZ_CP083948:c37446-37180 [Acinetobacter johnsonii]
ATGCAAGTATTTACATATACAGATGCACGTAACAATCTAAAATCCGTTTTAGATAAGGTTATTGATGATGCAGATGTAGC
AGTAATTACTAGGAAAGAAGGTAGTCATGCCGTTGTTATGGGGCAAGAACACTATGACAGCTTAATGGAAACTTTATATC
TTCTTTCATCTCCTAATAATGCTGCTCGTTTAAATGAGTCAATTGCCCAATTACGTGCATCCCGTACTACTGAAAGGGAT
CTCATCGAAGATGAATCGGACAATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT178332 WP_000554757.1 NZ_CP063473:885822-886115 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT178332 NZ_CP083948:c37196-36936 [Acinetobacter johnsonii]
ATGAATCGGACAATTGAGTTTACAGATAACGCTTGGGATGATTATATCTACTGGCAAACGCAGGATAAAAAGGTACTCAA
GCGTATAAATGCTTTAATCAAAGAGTGCCTACGAACACCATTTGAAGGAACAGGAAAGCCCGAACCACTTAAGGCGAACC
TTTCAGGCTTTTGGAGTCGAAGGATAGATGAAAAGCATAGGTTGGTTTATGAAGTTACGGATGAACGGATTAGCATCGTT
CAGTGTCGGTTTCACTACTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6