Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 879786..880327 Replicon chromosome
Accession NZ_CP063473
Organism Escherichia coli strain EM18-18-42

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag IQS24_RS04115 Protein ID WP_000615979.1
Coordinates 879786..880064 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag IQS24_RS04120 Protein ID WP_000729704.1
Coordinates 880067..880327 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS24_RS04100 877368..877946 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
IQS24_RS04105 878152..878919 + 768 WP_000333379.1 class II glutamine amidotransferase -
IQS24_RS04110 878890..879630 - 741 WP_001225679.1 murein L,D-transpeptidase -
IQS24_RS04115 879786..880064 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IQS24_RS04120 880067..880327 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IQS24_RS04125 880513..881286 + 774 WP_001353765.1 C40 family peptidase -
IQS24_RS04130 881462..881959 + 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
IQS24_RS04135 882175..883887 - 1713 Protein_794 flagellar biosynthesis protein FlhA -
IQS24_RS04140 883859..884644 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 871268..888724 17456


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T178331 WP_000615979.1 NZ_CP063473:c880064-879786 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T178331 NZ_CP083948:c37196-36936 [Acinetobacter johnsonii]
ATGAATCGGACAATTGAGTTTACAGATAACGCTTGGGATGATTATATCTACTGGCAAACGCAGGATAAAAAGGTACTCAA
GCGTATAAATGCTTTAATCAAAGAGTGCCTACGAACACCATTTGAAGGAACAGGAAAGCCCGAACCACTTAAGGCGAACC
TTTCAGGCTTTTGGAGTCGAAGGATAGATGAAAAGCATAGGTTGGTTTATGAAGTTACGGATGAACGGATTAGCATCGTT
CAGTGTCGGTTTCACTACTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT178331 WP_000729704.1 NZ_CP063473:c880327-880067 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT178331 NZ_CP083948:c37446-37180 [Acinetobacter johnsonii]
ATGCAAGTATTTACATATACAGATGCACGTAACAATCTAAAATCCGTTTTAGATAAGGTTATTGATGATGCAGATGTAGC
AGTAATTACTAGGAAAGAAGGTAGTCATGCCGTTGTTATGGGGCAAGAACACTATGACAGCTTAATGGAAACTTTATATC
TTCTTTCATCTCCTAATAATGCTGCTCGTTTAAATGAGTCAATTGCCCAATTACGTGCATCCCGTACTACTGAAAGGGAT
CTCATCGAAGATGAATCGGACAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References