Detailed information of TA system TA178320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2401132..2401826
• Replicon: chromosome
• Accession: NZ_CP063467
• Organism: Escherichia coli strain EM20-18-50

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IQS25_RS11620
• Protein ID: WP_001263493.1
• Coordinates: 2401132..2401530 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IQS25_RS11625
• Protein ID: WP_000554757.1
• Coordinates: 2401533..2401826 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS25_RS11590	2396132..2397376	-	1245	WP_000189541.1	esterase FrsA	-
-	2396792..2396872	-	81	NuclAT_11	-	-
-	2396792..2396872	-	81	NuclAT_11	-	-
-	2396792..2396872	-	81	NuclAT_11	-	-
-	2396792..2396872	-	81	NuclAT_11	-	-
IQS25_RS11595	2397468..2397926	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IQS25_RS11600	2398187..2399644	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
IQS25_RS11605	2399701..2400222	-	522	Protein_2270	peptide chain release factor H	-
IQS25_RS11610	2400221..2400424	-	204	Protein_2271	RNA ligase RtcB family protein	-
IQS25_RS11615	2400670..2401122	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
IQS25_RS11620	2401132..2401530	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IQS25_RS11625	2401533..2401826	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IQS25_RS11630	2401878..2402933	-	1056	WP_203139903.1	DNA polymerase IV	-
IQS25_RS11635	2403004..2403789	-	786	WP_000207549.1	putative lateral flagellar export/assembly protein LafU	-
IQS25_RS11640	2403761..2405473	+	1713	Protein_2277	flagellar biosynthesis protein FlhA	-
IQS25_RS11645	2405689..2406186	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T178320 WP_001263493.1 NZ_CP063467:c2401530-2401132 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T178320 NZ_CP083933:c235893-235450 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT178320 WP_000554757.1 NZ_CP063467:c2401826-2401533 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT178320 NZ_CP083933:c236120-235890 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6