Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 630662..631164 Replicon chromosome
Accession NZ_CP063467
Organism Escherichia coli strain EM20-18-50

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IQS25_RS02860 Protein ID WP_000767829.1
Coordinates 630910..631164 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IQS25_RS02855 Protein ID WP_001259255.1
Coordinates 630662..630913 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS25_RS02830 625840..626907 - 1068 WP_203139868.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
IQS25_RS02835 626907..627977 - 1071 WP_001468894.1 histidinol-phosphate transaminase -
IQS25_RS02840 627974..629278 - 1305 WP_203139870.1 histidinol dehydrogenase -
IQS25_RS02845 629284..630183 - 900 WP_000131776.1 ATP phosphoribosyltransferase -
IQS25_RS02850 630329..630379 - 51 WP_071791290.1 his operon leader peptide -
IQS25_RS02855 630662..630913 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IQS25_RS02860 630910..631164 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IQS25_RS02865 631247..632071 + 825 WP_000754737.1 SDR family oxidoreductase -
IQS25_RS02870 632117..633046 + 930 WP_024190947.1 LysR family transcriptional regulator -
IQS25_RS02875 633261..633323 + 63 WP_010723108.1 membrane protein YoeI -
IQS25_RS02880 633313..634671 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IQS25_RS02885 634850..635908 + 1059 WP_000492315.1 transport protein YeeE -
IQS25_RS02890 635922..636149 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T178308 WP_000767829.1 NZ_CP063467:630910-631164 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T178308 NZ_CP083931:1585973-1586254 [Alysiella filiformis DSM 16848]
ATGATTTTTGAATGGGACGAGAACAAGGCGGAAAGCAATTTCCGAAAACATGGCATCAGTTTCGCCAATGCTGCCAAAGT
TTTTGATGACCCAAATTGCATTACCCAACAAGACCGATTTGAAAATGGCGAATACCGTTGGCAAACAATCGGTATGGTTG
GTTCAGAACAATTATTGCTGGTCGCGCACACTTATCTTGATGATGATGAAAATACCGTTGTTCGCATTATTTCCGCGCGT
AAAGCAACCAAGCAGGAAAGGAAAAACTATGACAATCATTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT178308 WP_001259255.1 NZ_CP063467:630662-630913 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT178308 NZ_CP083931:1586241-1586537 [Alysiella filiformis DSM 16848]
ATGACAATCATTAGATACAAAGCCAACGAAATCCCCGAACCCACAGCAGAAGATATGGCGCGTATGGCAGCCATTCGTGA
TGAAGACATTGATTACAGCGACATTCCCCCTTTAACCGATGAATTTTGGGCAAATGCCAAACGGTTTAACGAACTGTATC
GCCCACAAAAACGACAAGTTACCGTGCGGATTGACGCTGATGTGTTGGCGTGGCTCAAACAAGATGGCAAAGGCTACCAA
ACACGTTTGAATGAAATTTTGCGACAAGCGATGTTGTCCACCAAATCGGCTGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References