Detailed information of TA system TA178303

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 15191..15455
• Replicon: plasmid pEM22-18-53_5
• Accession: NZ_CP063464
• Organism: Escherichia coli strain EM22-18-53

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: IQS26_RS24155
• Protein ID: WP_001331364.1
• Coordinates: 15191..15343 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	15398..15455 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS26_RS24120	11465..12161	-	697	Protein_13	IS1 family transposase	-
IQS26_RS24125	12217..12825	+	609	Protein_14	IncI1-type conjugal transfer membrane protein TraY	-
IQS26_RS24130	12901..13515	+	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
IQS26_RS24135	13613..13822	+	210	WP_001140545.1	hemolysin expression modulator Hha	-
IQS26_RS24140	14031..14207	+	177	WP_001054904.1	hypothetical protein	-
IQS26_RS24145	14272..14568	-	297	WP_000115013.1	hypothetical protein	-
IQS26_RS24150	14868..15119	+	252	WP_001535703.1	hypothetical protein	-
IQS26_RS24155	15191..15343	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	15398..15455	+	58	NuclAT_0	-	Antitoxin
-	15398..15455	+	58	NuclAT_0	-	Antitoxin
-	15398..15455	+	58	NuclAT_0	-	Antitoxin
-	15398..15455	+	58	NuclAT_0	-	Antitoxin
IQS26_RS24160	15635..16843	+	1209	WP_001535704.1	IncI1-type conjugal transfer protein TrbA	-
IQS26_RS24165	16862..17932	+	1071	WP_001535705.1	IncI1-type conjugal transfer protein TrbB	-
IQS26_RS24170	17925..20216	+	2292	WP_001289272.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA1 / ant(3'')-Ia / qacE / sul1 / tet(A)	-	1..94799	94799

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T178303 WP_001331364.1 NZ_CP063464:c15343-15191 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T178303 NZ_CP083930:c1535214-1534954 [Simonsiella muelleri]
ATGCGATTAACTTTTTCTGATGATGCATGGGACGATTATATTTTTTGGCAAACGACAGACCGAAAAATATTAAAACGCAT
CAACACATTAATTAAAGACATCAAACGCAACCCTTTTGACGGCATTGGTAAACCCGAACCCTTACGGTTTCATTTGTCAG
GCTTTTGGTCGCGTAGGATTGATGAAGAACATCGGTTGGTCTATGCAATTGAAAATGAACAAATTTTGATTGCTTCGTGT
CGGTATCATTACGTGGAATGA

Antitoxin

Nucleotide

• Length: 58 bp

>AT178303 NZ_CP063464:15398-15455 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin