Detailed information of TA system TA178298

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 104513..104938
• Replicon: plasmid pEM22-18-53_3
• Accession: NZ_CP063462
• Organism: Escherichia coli strain EM22-18-53

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: IQS26_RS23650
• Protein ID: WP_001332444.1
• Coordinates: 104777..104938 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	104513..104724 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS26_RS23625	100487..100984	+	498	WP_050427786.1	single-stranded DNA-binding protein	-
IQS26_RS23630	101047..101280	+	234	WP_001209608.1	DUF905 domain-containing protein	-
IQS26_RS23635	101339..103297	+	1959	Protein_90	ParB/RepB/Spo0J family partition protein	-
IQS26_RS23640	103352..103786	+	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
IQS26_RS23645	103783..104502	+	720	WP_001276275.1	plasmid SOS inhibition protein A	-
-	104513..104724	+	212	NuclAT_0	-	Antitoxin
-	104513..104724	+	212	NuclAT_0	-	Antitoxin
-	104513..104724	+	212	NuclAT_0	-	Antitoxin
-	104513..104724	+	212	NuclAT_0	-	Antitoxin
IQS26_RS23650	104777..104938	+	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
IQS26_RS23655	105176..105788	-	613	Protein_94	hypothetical protein	-
IQS26_RS23660	105967..106788	+	822	WP_001234485.1	DUF945 domain-containing protein	-
IQS26_RS23665	107084..107674	-	591	WP_000252676.1	transglycosylase SLT domain-containing protein	-
IQS26_RS23670	108008..108391	+	384	WP_000124981.1	relaxosome protein TraM	-
IQS26_RS23675	108581..109267	+	687	WP_000332490.1	PAS domain-containing protein	-
IQS26_RS23680	109361..109588	+	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..169293	169293
	-	flank	IS/Tn	-	-	99138..99641	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T178298 WP_001332444.1 NZ_CP063462:104777-104938 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T178298 NZ_CP083920:221905-222171 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Nucleotide

• Length: 212 bp

>AT178298 NZ_CP063462:104513-104724 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin