Detailed information of TA system TA178281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2232341..2233068
• Replicon: chromosome
• Accession: NZ_CP063459
• Organism: Escherichia coli strain EM22-18-53

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: IQS26_RS10740
• Protein ID: WP_000547564.1
• Coordinates: 2232341..2232652 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IQS26_RS10745
• Protein ID: WP_000126294.1
• Coordinates: 2232649..2233068 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS26_RS10710	2227483..2229192	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
IQS26_RS10715	2229202..2229744	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
IQS26_RS10720	2229744..2230511	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
IQS26_RS10725	2230508..2230918	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
IQS26_RS10730	2230911..2231381	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
IQS26_RS10735	2231406..2232167	+	762	WP_001026446.1	hypothetical protein	-
IQS26_RS10740	2232341..2232652	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
IQS26_RS10745	2232649..2233068	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
IQS26_RS10750	2233182..2234606	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
IQS26_RS10755	2234615..2236072	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
IQS26_RS10760	2236332..2237342	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
IQS26_RS10765	2237491..2238018	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T178281 WP_000547564.1 NZ_CP063459:2232341-2232652 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T178281 NZ_CP083911:c1471871-1471590 [Tepidimonas taiwanensis]
ATGCGCTATGAGCTTGCGTTTCACCCCGCCGCTTGGCGGGAGTGGCAAGCGCTCGACGGCAGCGTTCGCACGATCCTCAA
GCGCAAGCTCGCCGAGCGTCTCGAGCAGCCCCGGGTGCCGGCCGCGCAGCTGGCAGGGCATCCGGACCGTTACAAAATCA
AGCTGCGCAGCGCCGGTTACCGTCTCGTGTACGAGGTGCGGGACGCCGAGGTGGTGGTCATCGTGATCGCCGTGGGCAAG
CGCGAGCGCAGCGCCGTGTACCGGGCCGCCGACAGGCGTTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT178281 WP_000126294.1 NZ_CP063459:2232649-2233068 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT178281 NZ_CP083911:c1472115-1471861 [Tepidimonas taiwanensis]
ATGCCCCACGTGTTGCTCGCCCCCGTTGCGGCCAGCGTCACCGAACTCAAGCGCGATCCGATGGGCACGGTTCAGGCGGG
TGAGGGGGGGCCGGTGGCGATCCTCAATCGCAACGAGCCCGCCTTCTACTGCGTGCCCGCCACGGCTTTCGAGGCCTTGA
TGGAGCGTCTGGAGGATCTGGAGCTCAACGCCCTCGCGGACGCCCGGCTGCGGGACGGACAACCCTTGCAGCGGGTGTCC
CTCGATGCGCTATGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5