Detailed information of TA system TA178258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 4042728..4043428
• Replicon: chromosome
• Accession: NZ_CP063454
• Organism: Pseudomonas putida strain PC-578038

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: IP585_RS18500
• Protein ID: WP_023389615.1
• Coordinates: 4043132..4043428 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: A0A2L1I9E4
• Locus tag: IP585_RS18495
• Protein ID: WP_019437852.1
• Coordinates: 4042728..4043129 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IP585_RS18475	4037797..4038618	-	822	WP_014590266.1	transporter substrate-binding domain-containing protein	-
IP585_RS18480	4038694..4039635	-	942	WP_267273284.1	FAD-binding protein	-
IP585_RS18485	4039637..4040386	-	750	WP_014590265.1	electron transfer flavoprotein subunit beta/FixA family protein	-
IP585_RS18490	4040940..4042604	+	1665	WP_014590264.1	electron transfer flavoprotein-ubiquinone oxidoreductase	-
IP585_RS18495	4042728..4043129	-	402	WP_019437852.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
IP585_RS18500	4043132..4043428	-	297	WP_023389615.1	type II toxin-antitoxin system MqsR family toxin	Toxin
IP585_RS18505	4043523..4044848	-	1326	WP_267273285.1	MFS transporter	-
IP585_RS18510	4044921..4045400	-	480	WP_019437855.1	hypothetical protein	-
IP585_RS18515	4045569..4046045	-	477	WP_064493134.1	sigma-70 family RNA polymerase sigma factor	-
IP585_RS18520	4046259..4047437	+	1179	WP_064493133.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-97)

Antitoxin

• Antitoxin MqsA/HigA-2 (1-131)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11165.97 Da
• Isoelectric Point: 8.8385

>T178258 WP_023389615.1 NZ_CP063454:c4043428-4043132 [Pseudomonas putida]
MEKRTPHCPLERVRALVGAGRISPTTASLRGAQALGMEYADMLEVITGLERRDFYKSMTSYADHRVWQDVYRPLTVKGYL
YLKLSVVDDALIVSFKEL

Nucleotide

• Length: 297 bp

>T178258 NZ_CP083888:1819647-1819901 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14818.86 Da
• Isoelectric Point: 4.6580

>AT178258 WP_019437852.1 NZ_CP063454:c4043129-4042728 [Pseudomonas putida]
MRCPICGGAELALDTQDMPYRYKGESTLIPDVTGDYCSVCGEAVLSHDEAMRVSDLMSAFNRQVNALDVDPDFIVSVRKK
FDLDQREAGEIFGGGVNAFSRYENGKTKPPVALIKLFKLLDRHPELFEEVRTA

Nucleotide

• Length: 402 bp

>AT178258 NZ_CP083888:1819399-1819650 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2L1I9E4