Detailed information of TA system TA178239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1600322..1600982
• Replicon: chromosome
• Accession: NZ_CP063441
• Organism: Enterobacter asburiae strain SD4L

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: INS04_RS07405
• Protein ID: WP_126330275.1
• Coordinates: 1600322..1600675 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MX79
• Locus tag: INS04_RS07410
• Protein ID: WP_029741460.1
• Coordinates: 1600680..1600982 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INS04_RS07385	1596652..1597569	+	918	WP_047652425.1	nitrogen assimilation transcriptional regulator NAC	-
INS04_RS07390	1597666..1598616	+	951	WP_047652424.1	HTH-type transcriptional regulator Cbl	-
INS04_RS07395	1598660..1599268	-	609	Protein_1432	glutathione S-transferase family protein	-
INS04_RS07400	1599470..1600144	+	675	Protein_1433	transcriptional regulator	-
INS04_RS07405	1600322..1600675	+	354	WP_126330275.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
INS04_RS07410	1600680..1600982	+	303	WP_029741460.1	XRE family transcriptional regulator	Antitoxin
INS04_RS07415	1601058..1601978	-	921	WP_193806356.1	LysR family transcriptional regulator	-
INS04_RS07420	1602120..1602509	+	390	WP_059445031.1	RidA family protein	-
INS04_RS07430	1602939..1605128	-	2190	WP_193806358.1	TonB-dependent siderophore receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13561.45 Da
• Isoelectric Point: 9.0310

>T178239 WP_126330275.1 NZ_CP063441:1600322-1600675 [Enterobacter asburiae]
VWAINTTDRFDRWFTSLDDTERASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRMGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLNTLKERE

Nucleotide

• Length: 354 bp

>T178239 NZ_CP083886:1863265-1863516 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11050.83 Da
• Isoelectric Point: 5.7316

>AT178239 WP_029741460.1 NZ_CP063441:1600680-1600982 [Enterobacter asburiae]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT178239 NZ_CP083886:1863513-1863767 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MX79