Detailed information of TA system TA178191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 244366..244999
• Replicon: chromosome
• Accession: NZ_CP063421
• Organism: Vibrio cholerae strain VC_hf7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: INP70_RS15290
• Protein ID: WP_000843588.1
• Coordinates: 244366..244698 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: INP70_RS15295
• Protein ID: WP_000071008.1
• Coordinates: 244685..244999 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INP70_RS15255	240141..240629	+	489	WP_000424243.1	hypothetical protein	-
INP70_RS15260	240685..240792	+	108	WP_193836681.1	acetyltransferase	-
INP70_RS15265	241217..241732	+	516	WP_000100089.1	ClbS/DfsB family four-helix bundle protein	-
INP70_RS15270	242221..242487	+	267	WP_002022569.1	hypothetical protein	-
INP70_RS15275	242624..243238	+	615	WP_193836683.1	DUF2971 domain-containing protein	-
INP70_RS15280	243413..243649	-	237	WP_000772131.1	RNA-binding protein	-
INP70_RS15285	243914..244189	+	276	WP_000436062.1	hypothetical protein	-
INP70_RS15290	244366..244698	+	333	WP_000843588.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
INP70_RS15295	244685..244999	+	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
INP70_RS15300	245303..245473	+	171	Protein_286	DUF645 family protein	-
INP70_RS15305	245680..246027	+	348	WP_000933405.1	hypothetical protein	-
INP70_RS15310	246147..246602	+	456	WP_001245331.1	GNAT family N-acetyltransferase	-
INP70_RS15315	246809..247312	+	504	WP_000734953.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	dfrA31	-	152411..247312	94901
	-	inside	Integron	-	-	153082..246602	93520

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13035.98 Da
• Isoelectric Point: 9.9244

>T178191 WP_000843588.1 NZ_CP063421:244366-244698 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRSGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T178191 NZ_CP083877:c2405545-2405369 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT178191 WP_000071008.1 NZ_CP063421:244685-244999 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT178191 NZ_CP083877:c2405323-2404907 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA