Detailed information of TA system TA178127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5602792..5603387
• Replicon: chromosome
• Accession: NZ_CP063393
• Organism: Pseudomonas aeruginosa strain ST167_d67burn2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: IPV41_RS26000
• Protein ID: WP_003113526.1
• Coordinates: 5603109..5603387 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IPV41_RS25995
• Protein ID: WP_003113527.1
• Coordinates: 5602792..5603097 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPV41_RS25970 (IPV41_25975)	5597950..5598285	+	336	WP_034035738.1	hypothetical protein	-
IPV41_RS25975 (IPV41_25980)	5598295..5598684	+	390	WP_096219661.1	(deoxy)nucleoside triphosphate pyrophosphohydrolase	-
IPV41_RS25980 (IPV41_25985)	5598677..5599501	+	825	WP_096219662.1	HNH endonuclease	-
IPV41_RS25985 (IPV41_25990)	5599877..5601637	-	1761	WP_132764182.1	HEPN domain-containing protein	-
IPV41_RS25990 (IPV41_25995)	5602042..5602455	-	414	WP_049876905.1	hypothetical protein	-
IPV41_RS25995 (IPV41_26000)	5602792..5603097	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
IPV41_RS26000 (IPV41_26005)	5603109..5603387	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IPV41_RS26005	5603440..5603568	-	129	Protein_5148	integrase	-
IPV41_RS26010 (IPV41_26010)	5603716..5605944	+	2229	WP_023086667.1	TonB-dependent receptor	-
IPV41_RS26015 (IPV41_26015)	5606014..5606661	-	648	WP_003095021.1	carbonate dehydratase	-
IPV41_RS26020 (IPV41_26020)	5606723..5607961	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T178127 WP_003113526.1 NZ_CP063393:c5603387-5603109 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T178127 NZ_CP083862:c1051628-1051410 [Enterobacter kobei]
ATGTCTGAAAAACCACTCACAAAGGTCGATTATTTGATGCGCTTACGCCGTTGTCAGTCAATTGACACCCTCGAACGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCTGACCATCGCCTCGCTGAGC
TAACGATGAATAAGTTATATGACAAAATTCCCACATCCGTGTGGAAATTTGTACGTTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT178127 WP_003113527.1 NZ_CP063393:c5603097-5602792 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT178127 NZ_CP083862:c1052030-1051656 [Enterobacter kobei]
ATGGACGAGTACTCGCCAAAACGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGATCCAACGTCTGCCATCAATTTACAGTTGAACGAGCTTATCGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCGCAAGATTTGCAAAAATGGCGCAAATCGGGAAACCGACT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCCGTTAGCCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: V6ALY3