Detailed information of TA system TA178115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5602805..5603400
• Replicon: chromosome
• Accession: NZ_CP063391
• Organism: Pseudomonas aeruginosa strain ST167_d68blood2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: IPV43_RS26005
• Protein ID: WP_003113526.1
• Coordinates: 5603122..5603400 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IPV43_RS26000
• Protein ID: WP_003113527.1
• Coordinates: 5602805..5603110 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPV43_RS25975 (IPV43_25975)	5597963..5598298	+	336	WP_034035738.1	hypothetical protein	-
IPV43_RS25980 (IPV43_25980)	5598308..5598697	+	390	WP_096219661.1	(deoxy)nucleoside triphosphate pyrophosphohydrolase	-
IPV43_RS25985 (IPV43_25985)	5598690..5599514	+	825	WP_096219662.1	HNH endonuclease	-
IPV43_RS25990 (IPV43_25990)	5599890..5601650	-	1761	WP_132764182.1	HEPN domain-containing protein	-
IPV43_RS25995 (IPV43_25995)	5602055..5602468	-	414	WP_049876905.1	hypothetical protein	-
IPV43_RS26000 (IPV43_26000)	5602805..5603110	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
IPV43_RS26005 (IPV43_26005)	5603122..5603400	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IPV43_RS26010	5603453..5603581	-	129	Protein_5149	integrase	-
IPV43_RS26015 (IPV43_26010)	5603729..5605957	+	2229	WP_023086667.1	TonB-dependent receptor	-
IPV43_RS26020 (IPV43_26015)	5606027..5606674	-	648	WP_003095021.1	carbonate dehydratase	-
IPV43_RS26025 (IPV43_26020)	5606736..5607974	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T178115 WP_003113526.1 NZ_CP063391:c5603400-5603122 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T178115 NZ_CP083857:c63948-63598 [Enterobacter kobei]
ATGTGGACGGTACTGTTCGGAAAGGTGTTTGAGCTATGGTTGCTTGAACAGGAAGAAGGGTTACAGGAAAAGGTACTGGC
AGACATGCTAAATCTACAAAACTATGGACCTCGTCTTCCTCGTCCTTATGCCGATACGGTAAAAGGCTCGCAATATAACA
ATATGAAAGAGTTACGTATTCAATACGCTGGCCGTCCGATTCGTGCTTTATTCGCATTTGACCCCGAACGTCAGGCGATT
GTGCTTTGCGCAGGCAACAAGAGTAACGACAAGGCATTTTATGAAAAACTCATTCGCATTGCAGATGATGAATTTACCCG
GCATCTGACAAGCCTGGAGACAGCCAAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT178115 WP_003113527.1 NZ_CP063391:c5603110-5602805 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT178115 NZ_CP083857:c63601-63302 [Enterobacter kobei]
GTGAAAACCCTAAATGAATTAATTACATCACGCTCACCGGAAAGCCAACAACGAATTAAAGAAATGGCAGAGGAAATGAT
CCTTGAAACGGGTCTGCAACTTTTACGTGAAGAGCTCAGACTTTCGCAAAAAAATGTTGCCGAGCTAATGGGGGTGAGCC
AGCCCGCAGTAACACAAATAGAGCAACGTGGAAATGAACTAAAAATTGCCACTCTTAAGCGCTACATTGAGGCAATGGGT
GGGAAACTTTGTATTGACGTTGAACTTCCAACAGGAAAACGCGTTGCGTTTCATATCTAA

Structures

Toxin

• AlphaFold DB: V6ALY3