Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5616331..5616926 Replicon chromosome
Accession NZ_CP063387
Organism Pseudomonas aeruginosa strain ST1076_d100blood2

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag IPV47_RS26250 Protein ID WP_003113526.1
Coordinates 5616648..5616926 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag IPV47_RS26245 Protein ID WP_003111575.1
Coordinates 5616331..5616636 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IPV47_RS26220 (IPV47_26215) 5611343..5611615 - 273 WP_003115921.1 hypothetical protein -
IPV47_RS26225 (IPV47_26220) 5612390..5614276 + 1887 WP_071550095.1 ATP-binding protein -
IPV47_RS26230 5614455..5614727 + 273 WP_071550096.1 DNA-binding protein -
IPV47_RS26235 (IPV47_26225) 5614783..5615115 + 333 WP_024947075.1 hypothetical protein -
IPV47_RS26240 (IPV47_26230) 5615375..5615989 + 615 WP_123794473.1 hypothetical protein -
IPV47_RS26245 (IPV47_26235) 5616331..5616636 - 306 WP_003111575.1 HigA family addiction module antitoxin Antitoxin
IPV47_RS26250 (IPV47_26240) 5616648..5616926 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IPV47_RS26255 5616979..5617107 - 129 Protein_5198 integrase -
IPV47_RS26260 (IPV47_26245) 5617255..5619483 + 2229 WP_031686603.1 TonB-dependent receptor -
IPV47_RS26265 (IPV47_26250) 5619554..5620201 - 648 WP_003095021.1 carbonate dehydratase -
IPV47_RS26270 (IPV47_26255) 5620263..5621501 - 1239 WP_031686604.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T178091 WP_003113526.1 NZ_CP063387:c5616926-5616648 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T178091 NZ_CP083844:56147-56434 [Enterobacter asburiae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT178091 WP_003111575.1 NZ_CP063387:c5616636-5616331 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp

>AT178091 NZ_CP083844:55908-56147 [Enterobacter asburiae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References