Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4253079..4253806 Replicon chromosome
Accession NZ_CP063369
Organism Escherichia coli strain 1409

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag FOI11_RS20335 Protein ID WP_000550189.1
Coordinates 4253079..4253393 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag FOI11_RS20340 Protein ID WP_000560266.1
Coordinates 4253390..4253806 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOI11_RS20315 4249237..4250223 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
FOI11_RS20320 4250302..4250994 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
FOI11_RS20325 4251071..4251574 - 504 WP_001333820.1 M48 family metallopeptidase -
FOI11_RS20330 4251659..4252795 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
FOI11_RS20335 4253079..4253393 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
FOI11_RS20340 4253390..4253806 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
FOI11_RS20345 4253851..4255869 - 2019 WP_182125912.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
FOI11_RS20350 4256295..4258646 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T178042 WP_000550189.1 NZ_CP063369:4253079-4253393 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T178042 NZ_CP083821:c3278403-3277918 [Enterobacter cloacae subsp. cloacae]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT178042 WP_000560266.1 NZ_CP063369:4253390-4253806 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT178042 NZ_CP083821:c3278657-3278391 [Enterobacter cloacae subsp. cloacae]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References