Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2577386..2577927 Replicon chromosome
Accession NZ_CP063369
Organism Escherichia coli strain 1409

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag FOI11_RS12495 Protein ID WP_000615983.1
Coordinates 2577649..2577927 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag FOI11_RS12490 Protein ID WP_000729703.1
Coordinates 2577386..2577646 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOI11_RS12470 2573061..2573846 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
FOI11_RS12475 2573791..2575530 + 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
FOI11_RS12480 2575754..2576251 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FOI11_RS12485 2576427..2577176 - 750 WP_024192679.1 C40 family peptidase -
FOI11_RS12490 2577386..2577646 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FOI11_RS12495 2577649..2577927 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FOI11_RS12500 2578083..2578823 + 741 WP_001225679.1 murein L,D-transpeptidase -
FOI11_RS12505 2578794..2579561 - 768 WP_000333380.1 class II glutamine amidotransferase -
FOI11_RS12510 2579767..2580345 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T178039 WP_000615983.1 NZ_CP063369:2577649-2577927 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T178039 NZ_CP083821:c1093528-1093310 [Enterobacter cloacae subsp. cloacae]
ATGTCTGATAAACCACTCACAAAAACCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACACTCGAACGCGT
GATTGAAAAAAATAAATACGAGCTTTCTGATAATGAACTGACCGTATTTTATTCAGCCGCTGACCATCGCCTTGCCGAGC
TGACCATGAATAAACTGTATGACAAAATCCCGCCTTCCGTGTGGAAATTTGTCCGTTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT178039 WP_000729703.1 NZ_CP063369:2577386-2577646 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT178039 NZ_CP083821:c1093930-1093556 [Enterobacter cloacae subsp. cloacae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAATCATGGCTGGGTGAACGATCCAACATCAGCCATCAATTTGCAATTGAATGAGTTGATAGAGC
ATATCGCGACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAACTGATTGAGCAAATTGACGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCGCAGGATTTGCAGAAATGGCGTAAATCTGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References