Detailed information of TA system TA177971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1376315..1376917
• Replicon: chromosome
• Accession: NZ_CP063349
• Organism: Escherichia coli strain BS15R-A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IN859_RS06640
• Protein ID: WP_000897305.1
• Coordinates: 1376315..1376626 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IN859_RS06645
• Protein ID: WP_000356397.1
• Coordinates: 1376627..1376917 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IN859_RS06615	1372229..1372828	+	600	WP_001295269.1	glucose-1-phosphatase	-
IN859_RS06620	1372822..1373694	+	873	WP_000920762.1	virulence factor BrkB family protein	-
IN859_RS06625	1373691..1374128	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
IN859_RS06630	1374173..1375114	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
IN859_RS06635	1375178..1376086	-	909	WP_001162704.1	alpha/beta hydrolase	-
IN859_RS06640	1376315..1376626	+	312	WP_000897305.1	hypothetical protein	Toxin
IN859_RS06645	1376627..1376917	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
IN859_RS06650	1377522..1377740	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
IN859_RS06655	1377959..1378201	+	243	WP_001086388.1	hypothetical protein	-
IN859_RS06660	1378430..1379410	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
IN859_RS06665	1379809..1380738	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
IN859_RS06670	1380735..1381370	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1378430..1379410	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T177971 WP_000897305.1 NZ_CP063349:1376315-1376626 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T177971 NZ_CP083790:4189094-4189312 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT177971 WP_000356397.1 NZ_CP063349:1376627-1376917 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT177971 NZ_CP083790:4188693-4189067 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7