Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 533424..534223 Replicon chromosome
Accession NZ_CP063349
Organism Escherichia coli strain BS15R-A

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag IN859_RS02595 Protein ID WP_000347267.1
Coordinates 533759..534223 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IN859_RS02590 Protein ID WP_001307405.1
Coordinates 533424..533759 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IN859_RS02575 529209..529979 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
IN859_RS02580 529995..531329 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
IN859_RS02585 531704..533275 + 1572 WP_001273741.1 galactarate dehydratase -
IN859_RS02590 533424..533759 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IN859_RS02595 533759..534223 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IN859_RS02600 534278..535087 - 810 WP_000072193.1 aga operon transcriptional regulator AgaR -
IN859_RS02605 535336..536616 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
IN859_RS02610 536639..537112 + 474 WP_153782026.1 PTS N-acetylgalactosamine transporter subunit IIB -
IN859_RS02615 537123..537902 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IN859_RS02620 537892..538770 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IN859_RS02625 538788..539222 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 522549..534223 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T177970 WP_000347267.1 NZ_CP063349:533759-534223 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T177970 NZ_CP083790:c4017412-4017095 [Klebsiella variicola]
ATATTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGCCAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTATGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT177970 WP_001307405.1 NZ_CP063349:533424-533759 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT177970 NZ_CP083790:c4017102-4016815 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References