Detailed information of TA system TA177962

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 75443..75869
• Replicon: plasmid pBS90R-A_2
• Accession: NZ_CP063343
• Organism: Escherichia coli strain BS90R-A

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IN877_RS23950
• Protein ID: WP_001312861.1
• Coordinates: 75443..75601 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	75645..75869 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IN877_RS23925	70811..71500	-	690	WP_039002187.1	conjugal transfer transcriptional regulator TraJ	-
IN877_RS23930	71687..72070	-	384	WP_001151524.1	relaxosome protein TraM	-
IN877_RS23935	72391..72993	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
IN877_RS23940	73290..74111	-	822	WP_001234469.1	DUF945 domain-containing protein	-
IN877_RS23945	74234..74521	-	288	WP_000107535.1	hypothetical protein	-
IN877_RS23950	75443..75601	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	75645..75869	-	225	NuclAT_0	-	Antitoxin
-	75645..75869	-	225	NuclAT_0	-	Antitoxin
-	75645..75869	-	225	NuclAT_0	-	Antitoxin
-	75645..75869	-	225	NuclAT_0	-	Antitoxin
IN877_RS23955	75681..75869	+	189	WP_001299721.1	hypothetical protein	-
IN877_RS23960	75881..76600	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
IN877_RS23965	76597..77031	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IN877_RS23970	77086..79044	-	1959	WP_039000998.1	ParB/RepB/Spo0J family partition protein	-
IN877_RS23975	79109..79342	-	234	WP_000005987.1	DUF905 family protein	-
IN877_RS23980	79399..79938	-	540	WP_032332913.1	single-stranded DNA-binding protein	-
IN877_RS23985	79964..80170	-	207	WP_000275853.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / tet(M)	-	1..117739	117739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T177962 WP_001312861.1 NZ_CP063343:c75601-75443 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T177962 NZ_CP083790:277802-278077 [Klebsiella variicola]
ATGGAGCAGCAGGTTTTATCTTTACGTAATAAACAGCGCCACACGCTGGAGCAACTGTTTAAAATCCCTGTGCCACAGGG
CATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGCGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCATCCTTCGCCGGATACTGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT177962 NZ_CP063343:c75869-75645 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin