Detailed information of TA system TA177958

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40134..40388
• Replicon: plasmid pBS90R-A_2
• Accession: NZ_CP063343
• Organism: Escherichia coli strain BS90R-A

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: IN877_RS23770
• Protein ID: WP_001351576.1
• Coordinates: 40134..40340 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	40327..40388 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IN877_RS23740	36176..36577	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
IN877_RS23745	36510..36767	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
IN877_RS23750	36860..37513	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
IN877_RS23755	38453..39310	-	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
IN877_RS23760	39303..39377	-	75	WP_061357229.1	RepA leader peptide Tap	-
IN877_RS23765	39609..39866	-	258	WP_001541895.1	replication regulatory protein RepA	-
IN877_RS23770	40134..40340	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	40327..40388	+	62	NuclAT_1	-	Antitoxin
-	40327..40388	+	62	NuclAT_1	-	Antitoxin
-	40327..40388	+	62	NuclAT_1	-	Antitoxin
-	40327..40388	+	62	NuclAT_1	-	Antitoxin
IN877_RS23775	40730..40942	-	213	WP_001541896.1	ANR family transcriptional regulator	-
IN877_RS23780	41077..41637	-	561	WP_000139310.1	fertility inhibition protein FinO	-
IN877_RS23785	41692..42435	-	744	WP_039002216.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / tet(M)	-	1..117739	117739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T177958 WP_001351576.1 NZ_CP063343:c40340-40134 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T177958 NZ_CP083784:c4656178-4655894 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGTGAATAAACGGCTCTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT177958 NZ_CP063343:40327-40388 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin