Detailed information of TA system TA177957

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4538328..4538930
• Replicon: chromosome
• Accession: NZ_CP063341
• Organism: Escherichia coli strain BS90R-A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IN877_RS21685
• Protein ID: WP_000897305.1
• Coordinates: 4538619..4538930 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IN877_RS21680
• Protein ID: WP_000356397.1
• Coordinates: 4538328..4538618 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IN877_RS21655	4534254..4535156	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
IN877_RS21660	4535153..4535788	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
IN877_RS21665	4535785..4536714	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
IN877_RS21670	4537044..4537286	-	243	WP_001086388.1	hypothetical protein	-
IN877_RS21675	4537505..4537723	-	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
IN877_RS21680	4538328..4538618	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
IN877_RS21685	4538619..4538930	-	312	WP_000897305.1	hypothetical protein	Toxin
IN877_RS21690	4539159..4540067	+	909	WP_001162704.1	alpha/beta hydrolase	-
IN877_RS21695	4540131..4541072	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
IN877_RS21700	4541117..4541554	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
IN877_RS21705	4541551..4542423	-	873	WP_000920762.1	virulence factor BrkB family protein	-
IN877_RS21710	4542417..4543016	-	600	WP_001295269.1	glucose-1-phosphatase	-
IN877_RS21715	4543115..4543900	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T177957 WP_000897305.1 NZ_CP063341:c4538930-4538619 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T177957 NZ_CP083784:c4575030-4574710 [Klebsiella pneumoniae]
ATGCACATTTCAACTGTACCGGCCACGGTGCCGGTTTCGTCACGCCTGTCGCCCGTACAGGTCTGGCAGCAACTGTTAAC
GTATCTGCTGGAACACCATTACGGCCTGACACTTAACGACACGCCATTTCAAGACGACGAGGCCATCCAAGAGCATATTG
AAGCTGGTATTACACTTGCTGATGCAGTGAATTTCCTGGTGGAACGCTATGAACTGGTACGCACTGACCGTAAGGGATTC
ACTTGGCAAGATCAAACACCATTCCTGACCGCCACCGATATTCTCAGAGCCAGGCGAGCTACCGGATTAATAAATACTTG
A

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT177957 WP_000356397.1 NZ_CP063341:c4538618-4538328 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT177957 NZ_CP083784:c4575387-4575064 [Klebsiella pneumoniae]
ATGCCATTCACACTATCCCATGGATGGGGGATACAAAGCGATATAACCCAACGATTTGGCGCAAGATTAGTTCAGGAAGG
CAACCGGCTGCATTACCTGGCTGACAGGGCTAGTTTGACGGGAAGCTTTAGCCCAGAGCAGCTACAAAGCCTGGAAAATG
CTTTCCCACTGTTTATAGAGCAGTTAGGAAATATGTTGCGTTCCGGTGAGCTAAATCCACAGCAGCAACATCAGATGACC
ATTCAGCTTACAGGTCTAACTTGTATAGCTGATACCCTTAGTAGCTGGGGCTACGTTTACCTCACTGTTTATCCCGCCCA
ATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7