Detailed information of TA system TA177930

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 96169..96595
• Replicon: plasmid pBS74R-D_5
• Accession: NZ_CP063337
• Organism: Escherichia coli strain BS74R-D

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IN878_RS25375
• Protein ID: WP_001312861.1
• Coordinates: 96169..96327 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	96371..96595 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IN878_RS25340	91220..91447	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
IN878_RS25345	91541..92227	-	687	WP_000332484.1	PAS domain-containing protein	-
IN878_RS25350	92418..92801	-	384	WP_000124981.1	relaxosome protein TraM	-
IN878_RS25355	93078..93725	+	648	WP_000614934.1	transglycosylase SLT domain-containing protein	-
IN878_RS25360	94022..94843	-	822	WP_021573168.1	DUF945 domain-containing protein	-
IN878_RS25365	94960..95247	-	288	WP_000107538.1	hypothetical protein	-
IN878_RS25370	95603..95815	+	213	WP_162491396.1	hypothetical protein	-
IN878_RS25375	96169..96327	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	96371..96595	-	225	NuclAT_0	-	Antitoxin
-	96371..96595	-	225	NuclAT_0	-	Antitoxin
-	96371..96595	-	225	NuclAT_0	-	Antitoxin
-	96371..96595	-	225	NuclAT_0	-	Antitoxin
IN878_RS25380	96607..97326	-	720	WP_021573167.1	plasmid SOS inhibition protein A	-
IN878_RS25385	97323..97757	-	435	WP_000845930.1	conjugation system SOS inhibitor PsiB	-
IN878_RS25390	97812..99770	-	1959	WP_021573166.1	ParB/RepB/Spo0J family partition protein	-
IN878_RS25395	99835..100068	-	234	WP_000006030.1	DUF905 family protein	-
IN878_RS25400	100124..100663	-	540	WP_009426937.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cseA / vat	1..115346	115346

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T177930 WP_001312861.1 NZ_CP063337:c96327-96169 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T177930 NZ_CP083769:18443-18859 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT177930 NZ_CP063337:c96595-96371 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin