Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3942665..3943359 Replicon chromosome
Accession NZ_CP063332
Organism Escherichia coli strain BS74R-D

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag IN878_RS19135 Protein ID WP_001263489.1
Coordinates 3942961..3943359 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag IN878_RS19130 Protein ID WP_000554758.1
Coordinates 3942665..3942958 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IN878_RS19110 3938297..3938794 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
IN878_RS19115 3939018..3940730 - 1713 Protein_3722 flagellar biosynthesis protein FlhA -
IN878_RS19120 3940702..3941487 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
IN878_RS19125 3941558..3942613 + 1056 WP_001226164.1 DNA polymerase IV -
IN878_RS19130 3942665..3942958 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
IN878_RS19135 3942961..3943359 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
IN878_RS19140 3943369..3943821 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
IN878_RS19145 3944139..3944345 + 207 Protein_3728 RtcB family protein -
IN878_RS19150 3944341..3944862 + 522 Protein_3729 peptide chain release factor H -
IN878_RS19155 3944919..3946376 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
IN878_RS19160 3946637..3947095 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 3947691..3947771 + 81 NuclAT_12 - -
- 3947691..3947771 + 81 NuclAT_12 - -
- 3947691..3947771 + 81 NuclAT_12 - -
- 3947691..3947771 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T177921 WP_001263489.1 NZ_CP063332:3942961-3943359 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T177921 NZ_CP083768:1758875-1758977 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT177921 WP_000554758.1 NZ_CP063332:3942665-3942958 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT177921 NZ_CP083768:c1758984-1758839 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References