Detailed information of TA system TA177868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2264473..2265133
• Replicon: chromosome
• Accession: NZ_CP063301
• Organism: Klebsiella grimontii strain M5a1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A285B6D8
• Locus tag: INR80_RS10670
• Protein ID: WP_004132737.1
• Coordinates: 2264473..2264826 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A9E1DQ94
• Locus tag: INR80_RS10675
• Protein ID: WP_004132739.1
• Coordinates: 2264831..2265133 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INR80_RS10645	2259857..2260624	-	768	WP_004132727.1	molybdopterin-dependent oxidoreductase	-
INR80_RS10650	2260614..2261222	-	609	WP_004132729.1	cytochrome b/b6 domain-containing protein	-
INR80_RS10655	2261237..2261863	-	627	WP_004132731.1	hypothetical protein	-
INR80_RS10660	2262003..2262671	+	669	WP_004132733.1	heavy metal response regulator transcription factor	-
INR80_RS10665	2262671..2264089	+	1419	WP_004132735.1	heavy metal sensor histidine kinase	-
INR80_RS10670	2264473..2264826	+	354	WP_004132737.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
INR80_RS10675	2264831..2265133	+	303	WP_004132739.1	XRE family transcriptional regulator	Antitoxin
INR80_RS10680	2265327..2265530	+	204	Protein_2075	hypothetical protein	-
INR80_RS10685	2265531..2265864	+	334	Protein_2076	type II toxin-antitoxin system PemK/MazF family toxin	-
INR80_RS10695	2266188..2267645	+	1458	WP_004136541.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
INR80_RS10705	2268673..2270127	-	1455	WP_049089250.1	AMP nucleosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13554.49 Da
• Isoelectric Point: 10.0045

>T177868 WP_004132737.1 NZ_CP063301:2264473-2264826 [Klebsiella grimontii]
VWMIKTTDTFERWFTSFNDTDRASVLAALLVLREKGPGLSRPNADTLRGSRYSNMKELRIQSRGEPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMLPVAEREFTNWLKTFKEKE

Nucleotide

• Length: 354 bp

>T177868 NZ_CP083737:2479280-2479804 [Hafnia paralvei]
ATGCCAGATCTCGCTATTGAGATTTTTTCGAACGACGTCGAATATGATTTTGCTCATTTCGACTGTGGTGAAGAATCTCT
CAATATATTCCTCACCAATCATCTTGCCCGCCAGCACAACGCTCGAATCTTACGGGGTTATTTGCTGGTCACCAAAGAGC
CAAAACCTAAAGTCGTAGGATATTACACGTTTTCTGGCAGTTGCTTCGAGAAAGAAACGCTTCCTTCAAACACACAGAAA
CGGAAAGTACCTTACGCTAATGTGCCCAGCGTCACCTTGGGGCGACTGGCTATCCAAAAAGAGTTGCAGGGACAAGACTG
GGGAACAACGCTGGTAACTCATGCTATGAAAGTCATCTATCACGCGTCATTGGCGGTCGGCATCCATGGTATGTTTGTCG
ATGCTCTTAACGAAAAAGCCAAGCGGTTCTATCTTCAACTTGGTTTTATTGCGCTTACAGGCAATAACGCAAACTCGTTG
TTCTACCCAACAAAATCCATCGAAAAGCTGTTTGAAGACGAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11072.83 Da
• Isoelectric Point: 5.7424

>AT177868 WP_004132739.1 NZ_CP063301:2264831-2265133 [Klebsiella grimontii]
MGRTLEQLMADEKPEVVADARAMAADILLNIHLAELREKVQKTQVEMAQALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDVELPDGSHYGFEL

Nucleotide

• Length: 303 bp

>AT177868 NZ_CP083737:2479007-2479276 [Hafnia paralvei]
ATGTCCGCCCTAAAAAAACAACGTATCGATCTCAGACTCACCGATGATGACAAAAGCAAGATCGAAGAAGCGGCCGCAAT
GACCAACCAAACTATCACCCAGTTTATGGTTGCTAGCGCCGCTGAACGCGCAGAGGAAGTCATTGAGCAGCATCGTCGTC
TCGTTCTCAACGATGAGTCGTGGAATTTGGTGATGGACGCTATCAGCAATCCGCCAGCCCCAAACAGTCGGCTGAAACGA
GCATCGAAACGTCTAGCGAGCATGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A285B6D8