Detailed information of TA system TA177860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 58734..59461
• Replicon: plasmid pMAR14456-IncHI1B
• Accession: NZ_CP063278
• Organism: Klebsiella pneumoniae strain MAR14-456

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: IL253_RS26750
• Protein ID: WP_011251285.1
• Coordinates: 59150..59461 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IL253_RS26745
• Protein ID: WP_011251286.1
• Coordinates: 58734..59153 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IL253_RS26715	53902..54372	-	471	WP_048333570.1	hypothetical protein	-
IL253_RS26720	54565..55320	-	756	Protein_51	hypothetical protein	-
IL253_RS26725	55916..56359	+	444	WP_048333569.1	hypothetical protein	-
IL253_RS26730	56380..57168	+	789	WP_040217257.1	hypothetical protein	-
IL253_RS26735	57182..57517	+	336	Protein_54	conjugal transfer protein TraI	-
IL253_RS26740	57619..58587	-	969	WP_086642568.1	IS5 family transposase	-
IL253_RS26745	58734..59153	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
IL253_RS26750	59150..59461	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
IL253_RS26755	59666..60124	-	459	Protein_58	DDE-type integrase/transposase/recombinase	-
IL253_RS26760	60238..60935	+	698	Protein_59	IS1 family transposase	-
IL253_RS26765	60952..61386	+	435	Protein_60	thioredoxin fold domain-containing protein	-
IL253_RS26770	61403..61714	+	312	WP_011251282.1	hypothetical protein	-
IL253_RS26775	61728..62069	+	342	WP_011251281.1	hypothetical protein	-
IL253_RS26780	62129..63085	+	957	WP_011251280.1	DsbA family protein	-
IL253_RS26785	63202..63777	+	576	WP_074424927.1	hypothetical protein	-
IL253_RS26790	63885..64400	+	516	WP_041937820.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA	1..232362	232362
	-	inside	IScluster/Tn	-	-	47292..69603	22311

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T177860 WP_011251285.1 NZ_CP063278:c59461-59150 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T177860 NZ_CP083731:3401271-3401489 [Salmonella sp. A29-2]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT177860 WP_011251286.1 NZ_CP063278:c59153-58734 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT177860 NZ_CP083731:3400869-3401243 [Salmonella sp. A29-2]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1