Detailed information of TA system TA177825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 4559292..4560643
• Replicon: chromosome
• Accession: NZ_CP063241
• Organism: Pectobacterium brasiliense strain ZLMLSHJ5

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: IHJ54_RS20220
• Protein ID: WP_203449716.1
• Coordinates: 4559603..4560643 (+)
• Length: 347 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: -
• Locus tag: IHJ54_RS20215
• Protein ID: WP_039528006.1
• Coordinates: 4559292..4559606 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHJ54_RS20190	4555113..4556147	+	1035	WP_203449714.1	putative FMN-dependent luciferase-like monooxygenase	-
IHJ54_RS20195	4556144..4557277	+	1134	WP_203449715.1	alkylhydroperoxidase domain protein	-
IHJ54_RS20200	4557334..4557846	-	513	WP_164669160.1	phenolic acid decarboxylase	-
IHJ54_RS20205	4557946..4558818	+	873	WP_039556006.1	LysR family transcriptional regulator	-
IHJ54_RS20210	4558981..4559307	+	327	WP_014913674.1	helix-turn-helix domain-containing protein	-
IHJ54_RS20215	4559292..4559606	+	315	WP_039528006.1	HipA N-terminal domain-containing protein	Antitoxin
IHJ54_RS20220	4559603..4560643	+	1041	WP_203449716.1	HipA domain-containing protein	Toxin
IHJ54_RS20225	4560703..4561326	-	624	WP_203449717.1	LysE family translocator	-
IHJ54_RS20230	4561594..4562349	+	756	WP_203449718.1	thiol:disulfide interchange protein DsbG	-
IHJ54_RS20235	4562383..4563288	-	906	WP_203449719.1	siderophore-interacting protein	-
IHJ54_RS20240	4563542..4563793	+	252	WP_010281712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IHJ54_RS20245	4563790..4564077	+	288	WP_015842269.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IHJ54_RS20250	4564197..4565549	-	1353	WP_014913667.1	glutathione-disulfide reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (57-307)

Antitoxin

• HipA, N-terminal subdomain 1 (5-98)

Sequences

Toxin

Protein

• Length: 347 a.a.
• Molecular weight: 39596.58 Da
• Isoelectric Point: 6.5556

>T177825 WP_203449716.1 NZ_CP063241:4559603-4560643 [Pectobacterium brasiliense]
MTRCRILLTPLTHEEEIATGYSRKGLKHLAGKMHTSPNLRFTRQQFMHDLPQAQKGMSISGYQPKIQLVLEEQAFTVVDH
QGLYILKPSPIEFPHLAENEHATMTLMARLGFAVPPHGLLRFKPEQADDEPEFAFVIKRFDRDEKTGHPIHQEQLDGAMG
IGEKFGKIHTDGRQYVSYERLALFLSKHVNDNIVFKIDLFRRIAYAYMLGNNDMHLRNFGLMHSRSGQLMLAPIYDFVSV
APYPTYFSSCFMALPLLIQEEGDEALAPGFETAYGEYLGMDFILFGQRIGLSENLTKKLLADLTKEAECVETTYRDSFMP
ADAIETTLRCYRHRLNLMSILDAERI

Nucleotide

• Length: 1041 bp

>T177825 NZ_CP083702:100863-101279 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACATGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11899.72 Da
• Isoelectric Point: 9.2317

>AT177825 WP_039528006.1 NZ_CP063241:4559292-4559606 [Pectobacterium brasiliense]
MRRQVKVYLYGVHIGQLSQDDEGYLFEYKKSYIGPPLSLSLPIQTGTFRSKILPPYFASLAPEGWLRRQYSQLQHLDEKD
LFGILIQNGKNLIGAVQLIAEDKS

Nucleotide

• Length: 315 bp

>AT177825 NZ_CP083702:100636-100866 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA