Detailed information of TA system TA177819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 519436..519938
• Replicon: chromosome
• Accession: NZ_CP063241
• Organism: Pectobacterium brasiliense strain ZLMLSHJ5

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A7W2E5L4
• Locus tag: IHJ54_RS02240
• Protein ID: WP_039282578.1
• Coordinates: 519436..519690 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: IHJ54_RS02245
• Protein ID: WP_039282574.1
• Coordinates: 519687..519938 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHJ54_RS02220	514845..515684	+	840	WP_014913998.1	HTH-type transcriptional activator RhaS	-
IHJ54_RS02225	515709..516563	+	855	WP_039279287.1	helix-turn-helix domain-containing protein	-
IHJ54_RS02230	516753..518081	-	1329	Protein_430	AAA family ATPase	-
IHJ54_RS02235	518078..519060	-	983	Protein_431	RNA-directed DNA polymerase	-
IHJ54_RS02240	519436..519690	-	255	WP_039282578.1	Txe/YoeB family addiction module toxin	Toxin
IHJ54_RS02245	519687..519938	-	252	WP_039282574.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
IHJ54_RS02250	520014..521723	-	1710	WP_039504943.1	ABC transporter ATP-binding protein	-
IHJ54_RS02255	521720..522586	-	867	WP_010285293.1	ABC transporter permease	-
IHJ54_RS02260	522579..523538	-	960	WP_203449910.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10229.79 Da
• Isoelectric Point: 8.0353

>T177819 WP_039282578.1 NZ_CP063241:c519690-519436 [Pectobacterium brasiliense]
VKLIWSEEAWEDYLYWQDIDKRMVKKINELIKETRRTPFEGKGKPEPLKHNLAGFWSRRITEEHRLVYAITDDAMMIAAC
RYHY

Nucleotide

• Length: 255 bp

>T177819 NZ_CP083701:c4492153-4491779 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9340.61 Da
• Isoelectric Point: 4.6119

>AT177819 WP_039282574.1 NZ_CP063241:c519938-519687 [Pectobacterium brasiliense]
MRTISYSEARQNLSATMMKTVEDRAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANAKRLMSSIESLKAGNGEERD
IIE

Nucleotide

• Length: 252 bp

>AT177819 NZ_CP083701:c4492611-4492243 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W2E5L4