Detailed information of TA system TA177814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 25372..26044
• Replicon: chromosome
• Accession: NZ_CP063241
• Organism: Pectobacterium brasiliense strain ZLMLSHJ5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IHJ54_RS00115
• Protein ID: WP_039511562.1
• Coordinates: 25658..26044 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A433N287
• Locus tag: IHJ54_RS00110
• Protein ID: WP_010281167.1
• Coordinates: 25372..25671 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHJ54_RS00090	20637..21614	+	978	WP_203449763.1	nucleoside hydrolase	-
IHJ54_RS00095	21614..22591	+	978	WP_203449764.1	ABC transporter permease	-
IHJ54_RS00100	22588..23496	+	909	WP_039275990.1	ABC transporter permease	-
IHJ54_RS00105	23536..25335	+	1800	WP_203449765.1	ABC transporter ATP-binding protein	-
IHJ54_RS00110	25372..25671	-	300	WP_010281167.1	XRE family transcriptional regulator	Antitoxin
IHJ54_RS00115	25658..26044	-	387	WP_039511562.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IHJ54_RS00120	26366..27064	+	699	WP_005976717.1	D-galactonate utilization transcriptional regulator DgoR	-
IHJ54_RS00125	27061..27957	+	897	WP_040043799.1	2-dehydro-3-deoxygalactonokinase	-
IHJ54_RS00130	27941..28558	+	618	WP_040035632.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
IHJ54_RS00135	28555..29703	+	1149	WP_010281150.1	galactonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-113)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14779.76 Da
• Isoelectric Point: 4.7491

>T177814 WP_039511562.1 NZ_CP063241:c26044-25658 [Pectobacterium brasiliense]
MEVYRLAWEIVTRALFDTWFDEQTETVQEEILAHLAILEEDGPQLGRPQVDQIKGSKYKNMKELRVQVGGHPFRTFFAFD
RTRKAIVLCAGDKKGEDEKLFYERMIKIADAEFASHLTSPEVKEDGDA

Nucleotide

• Length: 387 bp

>T177814 NZ_CP083701:c3187886-3187713 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11048.08 Da
• Isoelectric Point: 10.6442

>AT177814 WP_010281167.1 NZ_CP063241:c25671-25372 [Pectobacterium brasiliense]
MATLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQTQLALALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT177814 NZ_CP083701:c3188210-3187914 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A433N287