Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/-
Location 5949719..5950374 Replicon chromosome
Accession NZ_CP063233
Organism Pseudomonas fluorescens strain KF1

Toxin (Protein)


Gene name higB Uniprot ID A0A855K4I9
Locus tag IM720_RS26800 Protein ID WP_024077334.1
Coordinates 5950030..5950374 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A855K566
Locus tag IM720_RS26795 Protein ID WP_024077333.1
Coordinates 5949719..5950033 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM720_RS26775 5945959..5946972 + 1014 WP_024077329.1 GGDEF domain-containing protein -
IM720_RS26780 5947190..5948440 + 1251 WP_024077330.1 aspartate aminotransferase family protein -
IM720_RS26785 5948437..5948601 - 165 Protein_5289 hypothetical protein -
IM720_RS26790 5948943..5949659 + 717 WP_024077332.1 hypothetical protein -
IM720_RS26795 5949719..5950033 - 315 WP_024077333.1 helix-turn-helix domain-containing protein Antitoxin
IM720_RS26800 5950030..5950374 - 345 WP_024077334.1 toxin HigB-2 Toxin
IM720_RS26805 5950468..5951559 - 1092 WP_150295902.1 pyridoxal phosphate-dependent aminotransferase -
IM720_RS26810 5951707..5952594 - 888 WP_024077336.1 heme o synthase -
IM720_RS26815 5952606..5952941 - 336 WP_003176003.1 cytochrome o ubiquinol oxidase subunit IV -
IM720_RS26820 5952941..5953567 - 627 WP_024077337.1 cytochrome o ubiquinol oxidase subunit III -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(42-94)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13327.19 Da        Isoelectric Point: 9.8860

>T177792 WP_024077334.1 NZ_CP063233:c5950374-5950030 [Pseudomonas fluorescens]
MRSIFFETSSFTATVGHYLTDDEYRLLQNEMQWNPLAGDVMPRTGGFRKLRWSDGKRGKGKRSGLRVIYYWLTTDGHFWM
FAIYNKDELENLTADQEKALKKAIEAELKKRGTP

Download         Length: 345 bp

>T177792 NZ_CP083690:3632617-3633039 [Serratia sp. HRI]
GTGGCCCAGTGGCGATGTGATATCCGCATTTCCTGGCGCACTCAGCTGTTTTCCCTGTTGACCCACGGCGTTCTGATCCT
GCTGATCCTGATTTCGCCCTGGCCGGAAGGCTTTGGTCCGCTCTGGCTGGTGCTGCTGACGCTGGTGGTGTTTCAGTGCA
TTCGCAGCCAGAAGCGCATCGCCGCGGTGCAGGGCGAGCTGCGGCTGTTGGCGGACCGGCGCTTCAGCTGGCACGGCCGC
GAGTGGCGGCTGGCGAAAAAACCGTGGATGCCGGGTTACGGCATGCTGCTGACGCTGCAGCCGACGGAGGGCAAAAAGCG
CCGCCGGCTGTGGCTGGCTTCCGACTGCATGAGCAAAGAGGAGTGGCGCCACCTGCGGCAGCTGTTGCTGTATCCGCCGG
TGGGTGACGGCGAGGAGGCCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11789.63 Da        Isoelectric Point: 10.3067

>AT177792 WP_024077333.1 NZ_CP063233:c5950033-5949719 [Pseudomonas fluorescens]
MKKRDLFAELMQGVSDMTAHREGKITLRQSTVEDKPVPEVSAQEIVALRDKLHMSQSVFARSIRTSPGTLRNWEQDKSKP
NAQAALLIRLVEKFPDMVDRLATV

Download         Length: 315 bp

>AT177792 NZ_CP083690:3632370-3632636 [Serratia sp. HRI]
ATGGATATTAACAACAAAGCACGTATTCACTGGGCTTGCCGCCGTGGCATGCGCGAGCTGGACATCTCCATCATGCCGTT
CTTCGAATACGAATACGACAGCCTGAATGACGCGGACAAGGCGTTGTTCATCCGCCTGCTGGAGTGCGACGATCCCGATT
TGTTCAACTGGCTGATGAATCATGGCGCGCCGCAGGACAGCGAGCTGCAGAGAATGGTGACCCTGATCCAAACGCGAAAT
AAAGACCGTGGCCCAGTGGCGATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A855K4I9

Antitoxin

Source ID Structure
AlphaFold DB A0A855K566

References