Detailed information of TA system TA177784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4373549..4374201
• Replicon: chromosome
• Accession: NZ_CP063229
• Organism: Serratia marcescens strain LVF3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IM817_RS21020
• Protein ID: WP_004931830.1
• Coordinates: 4373549..4373893 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A086GAN0
• Locus tag: IM817_RS21025
• Protein ID: WP_004931828.1
• Coordinates: 4373899..4374201 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM817_RS21010 (IM817_21015)	4369891..4372149	-	2259	WP_219228460.1	molybdopterin guanine dinucleotide-containing S/N-oxide reductase	-
IM817_RS21015 (IM817_21020)	4372370..4373389	+	1020	WP_219228461.1	HTH-type transcriptional regulator GalR	-
IM817_RS21020 (IM817_21025)	4373549..4373893	+	345	WP_004931830.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IM817_RS21025 (IM817_21030)	4373899..4374201	+	303	WP_004931828.1	XRE family transcriptional regulator	Antitoxin
IM817_RS21030 (IM817_21035)	4374229..4375491	-	1263	WP_219228462.1	diaminopimelate decarboxylase	-
IM817_RS21035 (IM817_21040)	4375625..4376548	+	924	WP_219228464.1	LysR family transcriptional regulator	-
IM817_RS21040 (IM817_21045)	4376576..4377484	-	909	WP_219228466.1	LysR family transcriptional regulator	-
IM817_RS21045 (IM817_21050)	4377593..4378477	+	885	WP_033635778.1	MBL fold metallo-hydrolase	-
IM817_RS21050 (IM817_21055)	4378546..4379193	+	648	WP_219228467.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13383.44 Da
• Isoelectric Point: 10.6087

>T177784 WP_004931830.1 NZ_CP063229:4373549-4373893 [Serratia marcescens]
MWQVVTVERFDDWFLALNNAQQTSILAAIYKLQTFGPQLARPHADTLHFSDAVRQLKELRIQHRGRPFRVFFAFDPQRQA
VLLCGGDKTGDKRFYQRMLPIAAMEFSHYLATRR

Nucleotide

• Length: 345 bp

>T177784 NZ_CP083690:c859138-858884 [Serratia sp. HRI]
ATGAACAAACGACACCAAAAAACGCTGTCAGATGTGTTTGCCCGGCCTGTCAACGGTTCTATAAAGTGGTCTGATATTGA
GGCGCTTTTTACCGCATTAGGGGCGGAGATTCACGAAAGGGAAGGTTCTAGGATCGCGGTGCTGTTGAAAGGTGAAAAAA
GAGTCTTTCACCGGCCACACCCCAGACCTACCACTGACAAGGGGGCGGTTAACTCTATTCGGATCTGGTTGGATAGCTTA
GGAATAAAACCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11080.97 Da
• Isoelectric Point: 10.1998

>AT177784 WP_004931828.1 NZ_CP063229:4373899-4374201 [Serratia marcescens]
MAKPLSQLIDKLDPAVVAAARQKADQEIFELRLAMLREELAVSQVELAKRLGISQPSVANLEKRGSEIKLSSLKRYIEAM
GGTLSLDVQLPNGQHRRMTL

Nucleotide

• Length: 303 bp

>AT177784 NZ_CP083690:c858887-858543 [Serratia sp. HRI]
ATGATGAATAACACACTGAAAATTGACGGCCATACGGCCGTCATCAACTTCGACCCAGAAATTGAAATGTTCCGGGGCGA
GTTTGTCGGGCTGAACGGTGGCGCCGACTTCTACGCCTACAGCGTGGACGAGCTGAAGAAAGAAGGCGCGATCTCACTCG
CGGTCTTTCTCGATGAGTGCCATAAAGACGGCATCGAGCCTTACAAGTCGTACAGCGGCAAAGTAACCACCCGCCTGTCG
CCGGAACGCCATCAGGCGTTAGCCATTGCCGCTCAGGCCACCGGGCAGTCGATTAATGAACTGCTGAATGAAGGTGTTGA
TCTGGTTATCGAAAAGCATTCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A086GAN0