Detailed information of TA system TA177755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1259075..1259802
• Replicon: chromosome
• Accession: NZ_CP063224
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain BD-50-Eh

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IE977_RS05930
• Protein ID: WP_047718997.1
• Coordinates: 1259491..1259802 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IE977_RS05925
• Protein ID: WP_047718995.1
• Coordinates: 1259075..1259494 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE977_RS05910	1256149..1257270	-	1122	WP_152044016.1	glutamate--cysteine ligase	-
IE977_RS05915	1257376..1258266	+	891	WP_017694363.1	oxidoreductase	-
IE977_RS05920	1258317..1258751	-	435	WP_003858863.1	hypothetical protein	-
IE977_RS05925	1259075..1259494	-	420	WP_047718995.1	helix-turn-helix domain-containing protein	Antitoxin
IE977_RS05930	1259491..1259802	-	312	WP_047718997.1	type II toxin-antitoxin system HigB family toxin	Toxin
IE977_RS05935	1260163..1261992	-	1830	WP_195207140.1	ATP-binding protein	-
IE977_RS05940	1261989..1263032	-	1044	WP_047719000.1	SIR2 family protein	-
IE977_RS05945	1263340..1263795	-	456	WP_045142667.1	hypothetical protein	-
IE977_RS05950	1263822..1263929	-	108	WP_157843337.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-134)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12572.38 Da
• Isoelectric Point: 9.9435

>T177755 WP_047718997.1 NZ_CP063224:c1259802-1259491 [Enterobacter hormaechei subsp. steigerwaltii]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSMDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Nucleotide

• Length: 312 bp

>T177755 NZ_CP083651:c3425967-3425491 [Citrobacter amalonaticus]
ATGGATTTTCCCACATGGCTGAACGGGTGGACGATCTACGCTCATCCCTGTTTTATCGATCAATACAACGCGCTGGTCGC
ACGTGTTGAAGACCTTAAGCACAGGTTTCCAGATCCCATTGTTTTCCAGAAGAAGAAAGAGACCAAGGTGCTGGCGCATT
TATTGAAATCCATTGCCAACATTACCCACGAACCCCGAGCTTCTGTTTACCTGCCTGGTGACAGTATAGGGAAAGCTTAT
ACAGACTGGTCGAGAGCGAAGTTTGGTGGGGGACGGTATCGCTTATTTTTCCGTTATAGTCTCGAGAGTAAGATCATTGT
CATCGCATGGGTGAATGATGAAGGGCCATTGCGCACCTACGGCAGCAAGACCGATGCCTATAAGATTTTCGGGAAAATGC
TCGATGAGGGGAATCCACCCGACGACTGGCTAAGTTTGCTTCAGGCTTGTCAAAACGACGGGAAGGAACATCTTTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15407.49 Da
• Isoelectric Point: 4.7238

>AT177755 WP_047718995.1 NZ_CP063224:c1259494-1259075 [Enterobacter hormaechei subsp. steigerwaltii]
MNYAAAIKAANALTNVLPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESSEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Nucleotide

• Length: 420 bp

>AT177755 NZ_CP083651:c3426307-3425972 [Citrobacter amalonaticus]
ATGTTCGCCTATGTTCGATCTGAAGACATTGCCATTGAAGCGGATTCTAAATTGACGGAACGACATCAAACGACTATCCC
TGCGTCGATCAGGGACGCTTTACATCTTCGCAGTGGTGATCGTATTCATTACACATTGCTTTCCACGGGTGAAGTACTGC
TTTCAAAATACAGTGAAGCAACGGAGGACGGTGTGATGCGATCATTCCTGGACTTCTTATCACAGGATGTGGCGAATAAC
CCACAAACGCTGAAGAAGCTGGATTTATCCCGTGGCTATGAACTGGTTGATGGTATCGACGTCAATCTTGATGATGAAAT
CAGCGACGAAGACTGA