Detailed information of TA system TA177749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 46085..46812
• Replicon: plasmid pHKU57_1
• Accession: NZ_CP063216
• Organism: Klebsiella pneumoniae strain HKU57

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: IM695_RS25410
• Protein ID: WP_011251285.1
• Coordinates: 46085..46396 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IM695_RS25415
• Protein ID: WP_011251286.1
• Coordinates: 46393..46812 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM695_RS25385	42851..43030	-	180	Protein_49	IS5/IS1182 family transposase	-
IM695_RS25390	43070..44598	-	1529	Protein_50	IS66 family transposase	-
IM695_RS25395	44647..44994	-	348	WP_004114612.1	IS66 family insertion sequence element accessory protein TnpB	-
IM695_RS25400	44991..45329	-	339	WP_023288266.1	IS66 family insertion sequence hypothetical protein	-
IM695_RS25405	45422..45880	+	459	Protein_53	DDE-type integrase/transposase/recombinase	-
IM695_RS25410	46085..46396	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
IM695_RS25415	46393..46812	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
IM695_RS25420	46959..47222	+	264	Protein_56	transposase	-
IM695_RS25425	47332..48294	+	963	Protein_57	IS4-like element ISVsa5 family transposase	-
IM695_RS25430	48404..49612	+	1209	WP_001339197.1	IS4-like element ISVsa5 family transposase	-
IM695_RS25435	49618..50337	+	720	Protein_59	IS5 family transposase	-
IM695_RS25440	50406..50941	-	536	Protein_60	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / sul1 / qacE / dfrA25 / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / mph(E) / msr(E) / armA / blaDHA-1 / qnrB4	-	1..273789	273789
	-	inside	IScluster/Tn	-	-	42851..50337	7486

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T177749 WP_011251285.1 NZ_CP063216:46085-46396 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T177749 NZ_CP083648:2429294-2429602 [Corynebacterium coyleae]
GTGGAGATCATTTCCAGCAGCTTGTTTGACGCCTGGTTAGGTGAGCTAAAAGACAAACATGCTCAGCGACGCATTTTGTA
TGCAATAGCCCGATGCGAAGCACACGGAAGAATGATTGGAGACATCAAACCCGTTGGCGAGAAGGTTAGCGAGATGAGAT
TTCATTTCGGGCCCGGCTATCGGGTGTACTACACCCGACTAGGCGCAATAACGGTTTTCCTGCTGGCCGGAGGAGATAAA
TCCAGCCAGGATAAAGATATTCGAACCGCTCAAAAGCTCGCACAACAGGTAAGGGAGGAACAGTCGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT177749 WP_011251286.1 NZ_CP063216:46393-46812 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT177749 NZ_CP083648:2429599-2429892 [Corynebacterium coyleae]
GTGAAGGAAGTAACATTTTCAGCGTTCGATGCAAGCAAATATCTCGATAGCCAAGAAGCAGTGAACGATTACCTGGCTAT
CGCACTCGAAGACGGCGATACGAAAACCGTTCAGGTGGTTTTGCGTGACATTGCAAGGGCACACGGCATGAGCCAGCTCG
CTAAAGAGACTCAACTCAACCGTGAATCCCTCTACAAGTCACTATCAAAAGACGGTAACCCGTCCTTTGCGGCTATCACG
AAAATCATGAAAGCCCTGGGACTCAAAATAACTCTCGCTACACAAAATGCCTAG

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1