Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3815621..3816218 Replicon chromosome
Accession NZ_CP063215
Organism Klebsiella pneumoniae strain HKU57

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag IM695_RS18535 Protein ID WP_004142563.1
Coordinates 3815901..3816218 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag IM695_RS18530 Protein ID WP_004142561.1
Coordinates 3815621..3815908 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM695_RS18500 3811701..3811949 + 249 WP_020325243.1 DUF1158 domain-containing protein -
IM695_RS18505 3811967..3812308 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
IM695_RS18510 3812339..3813454 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
IM695_RS18515 3813634..3814215 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
IM695_RS18520 3814215..3814583 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
IM695_RS18525 3814703..3815356 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
IM695_RS18530 3815621..3815908 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
IM695_RS18535 3815901..3816218 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IM695_RS18540 3816403..3817446 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
IM695_RS18545 3818116..3818982 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
IM695_RS18550 3819091..3820518 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T177742 WP_004142563.1 NZ_CP063215:c3816218-3815901 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T177742 NZ_CP083643:c4039623-4039339 [Enterobacter bugandensis]
ATGATTTATGAACTGGAGTTCGACCCGAGGGCTTTAAAGGAGTGGCATAAGCTGGGCGATACGGTGAAATGCCAGTTTAA
AAAGAAACTTGCTGACGTCTTAGTGAACCCCAGAATTGAATCGGCCCGCCTGCACGGCCTGTCGGATTGCTACAAAATAA
AACTCAGATCTCTGGGTTATCGTTTGGTTTATCAGGTTCAGGATAACGTAGTTACGGTATTTGTTATCGCTATCGGAAAG
AGAGAGAAATCAGCCGTTTATCATGAAGCAAATAAACGGCTTTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT177742 WP_004142561.1 NZ_CP063215:c3815908-3815621 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT177742 NZ_CP083643:c4039855-4039613 [Enterobacter bugandensis]
ATGGCCACGCTTAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCGTATGCCGTGCTGGAAAAACTCAATATTACCCC
AACGGAAGCCGTGCGGTTACTGTTTCAGTACGTTGCGGAAACAGGGCGTATGCCGGTGAAAACTGTCACCATCAGTGACA
GTGAAGATGAACTGATACAGACAGTCCGGGAGCGACTGGCGAATCCACAAAAGGGAATTAAGGTTAGTCTGGATGATTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References