Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4636674..4637353 Replicon chromosome
Accession NZ_CP063214
Organism Escherichia coli strain ERP001

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag IL987_RS22230 Protein ID WP_000057523.1
Coordinates 4636674..4636976 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag IL987_RS22235 Protein ID WP_000806442.1
Coordinates 4637012..4637353 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IL987_RS22205 4632050..4633702 + 1653 WP_193579237.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
IL987_RS22210 4633740..4634243 - 504 WP_000667000.1 hypothetical protein -
IL987_RS22215 4634240..4635040 - 801 WP_000439798.1 hypothetical protein -
IL987_RS22220 4635064..4635543 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
IL987_RS22225 4635747..4636541 - 795 WP_000365148.1 TraB/GumN family protein -
IL987_RS22230 4636674..4636976 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IL987_RS22235 4637012..4637353 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
IL987_RS22240 4637411..4639915 - 2505 WP_000083943.1 copper-exporting P-type ATPase CopA -
IL987_RS22245 4640177..4641109 + 933 WP_000883025.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T177729 WP_000057523.1 NZ_CP063214:4636674-4636976 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T177729 NZ_CP083641:308513-308797 [Enterobacter bugandensis]
ATGATTTATGAACTGGAGTTCGACCCGAGGGCTTTAAAGGAGTGGCATAAGCTGGGCGATACGGTGAAATGCCAGTTTAA
AAAGAAACTTGCTGACGTCTTAGTGAACCCCAGAATTGAATCGGCCCGCCTGCACGGCCTGTCGGATTGCTACAAAATAA
AACTCAGATCTCTGGGTTATCGTTTGGTTTATCAGGTTCAGGATAACGTAGTTACGGTATTTGTTATCGCTATCGGAAAG
AGAGAGAAATCAGCCGTTTATCATGAAGCAAATAAACGGCTTTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT177729 WP_000806442.1 NZ_CP063214:4637012-4637353 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT177729 NZ_CP083641:308281-308523 [Enterobacter bugandensis]
ATGGCCACGCTTAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCGTATGCCGTGCTGGAAAAACTCAATATTACCCC
AACGGAAGCCGTGCGGTTACTGTTTCAGTACGTTGCGGAAACAGGGCGTATGCCGGTGAAAACTGTCACCATCAGTGACA
GTGAAGATGAACTGATACAGACAGTCCGGGAGCGACTGGCGAATCCACAAAAGGGAATTAAGGTTAGTCTGGATGATTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References