Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2537862..2538661 Replicon chromosome
Accession NZ_CP063214
Organism Escherichia coli strain ERP001

Toxin (Protein)


Gene name yhaV Uniprot ID Q8FDB4
Locus tag IL987_RS12345 Protein ID WP_000347252.1
Coordinates 2538197..2538661 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag IL987_RS12340 Protein ID WP_001296435.1
Coordinates 2537862..2538197 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IL987_RS12325 2533647..2534417 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
IL987_RS12330 2534433..2535767 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
IL987_RS12335 2536142..2537713 + 1572 WP_001273939.1 galactarate dehydratase -
IL987_RS12340 2537862..2538197 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IL987_RS12345 2538197..2538661 + 465 WP_000347252.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IL987_RS12350 2538716..2539525 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IL987_RS12355 2539774..2541054 + 1281 WP_000681930.1 tagatose-bisphosphate aldolase subunit KbaZ -
IL987_RS12360 2541077..2541550 + 474 WP_001298322.1 PTS N-acetylgalactosamine transporter subunit IIB -
IL987_RS12365 2541561..2542340 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IL987_RS12370 2542330..2543208 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IL987_RS12375 2543226..2543660 + 435 WP_000948837.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17750.11 Da        Isoelectric Point: 9.4947

>T177718 WP_000347252.1 NZ_CP063214:2538197-2538661 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Download         Length: 465 bp

>T177718 NZ_CP083638:3835070-3835222 [Escherichia fergusonii]
ATGCCGCAGAAATACGGATTACTTTCGTTAATAGTGATTTGTTTCACGCTTTTATTTTTCACCTGGATGATAAGAGATTC
ACTGTGTGAATTGCATATTAAACAGGAGAGTTATGAGCTGGCGGCGTTTTTAGCCTGCAAATTGAAAGAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT177718 WP_001296435.1 NZ_CP063214:2537862-2538197 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT177718 NZ_CP083638:c3835019-3834964 [Escherichia fergusonii]
GTTGAAGCATAGAGGCAGCCTCACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q8FDB4

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References